喉鳞癌组织中Oct-4 mRNA的表达及意义

目的探讨Oct-4 mRNA在喉鳞癌组织中的表达及意义。方法采用RT-PCR和荧光定量PCR检测48例喉鳞癌及其癌旁正常喉黏膜组织中Oct-4 mRNA的表达。结果 RT-PCR显示Oct-4 mRNA在喉鳞癌组织中的阳性率为64.6%,荧光定量PCR显示喉鳞癌组织中Oct-4 mRNA的相对表达量为0.533±0.142,而癌旁正常喉黏膜组织未检测出Oct-4基因的表达（P〈0.01）。Oct-4 mRNA在喉鳞癌高分化组阳性率为33.3%,显著低于中、低分化组的69.0%（P〈0.05）;无淋巴结转移组阳性率为47.8%,显著低于有淋巴结转移组的80.0%（P〈0.05）;Ⅰ、Ⅱ期组阳性率为47.4%,低于Ⅲ、Ⅳ期组的75.9%（P〈0.05）。荧光定量PCR结果显示Oct-4基因的相对表达量随着喉鳞癌组织分化减低、淋巴结转移和高的临床分期而显著增加（P〈0.05）。结论 Oct-4基因的高表达可能参与了喉鳞癌的发生、发展,并对判断喉鳞癌患者的生物学行为有一定意义。

Expression and Significance of Oct-4 Gene in Laryngeal Squamous Cell Carcinoma

Objective To investigate the expression and significance of Oct-4 mRNA in laryngeal squamous cell carcinoma. Methods RT-PCR and fluorescence quantitative PCR were used to detect Oct4 mRNA expressions in the 48 cases of laryngeal squamous cell carcinoma tissues and paraneoplastic nomal mucosa tissues. Results The result of RT-PCR showed that the positive rate of Oct4 mRNA was 64.6% in the laryngeal squamous cell carcino- ma tissues ,the result of fluorescence quantitative PCR showed that the amount of Oct-4mRNA in the laryngeal squa- mous cell carcinoma tissues was 0. 533± 0. 142, while no Oct-4 mRNA was found in the paraneoplastic normal mu- cosa tissues（ P 〈 0.05 ）. The expression rate of Oct-4 mRNA in the well differentiated laryngeal squamous cell carci- noma tissues（ 33.3% ） was lower than that in the medium and poor differentiated laryngeal squamous cell carcino- ma tissues（69.0% ） （ P 〈 0.05 ）. The expression rate of Oct-4 mRNA in the no lymph node metastasis group （47.8%） was lower than that in the lymph node metastasis group （80.0%） （P 〈 0.05 ）. The positive expression rate of Oct-4 mRNA in Ⅰ-Ⅱ phase（47.4% ） was lower than that in the Ⅲ-Ⅳ phase（75.9% ） （P 〈0.05）. The result of fluorescence quantitative PCR showed that the amount of Oct-4 mRNA in the laryngeal squamous cell carci- noma tissues was obviously increased with the lower differentiation, the lymph node metastasis and higher clinical stages. Conclusion The overexpression of Oct-4 mRNA may be associated with the carcinogenesis and development of laryngeal squamous cell carcinoma, and can be used for judging its biological behavior.