| 高效液相色谱法测定急麻颗粒中黄芩苷的含量 |
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| 引用本文： | 刘红,刘辉,刘彬果.高效液相色谱法测定急麻颗粒中黄芩苷的含量[J].天津药学,2014(6):11-12. |
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| 作者姓名： | 刘红 刘辉 刘彬果 |
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| 作者单位： | 中国人民解放军第二五四医院,天津300142 |
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| 摘 要： | 目的：建立高效液相色谱法测定急麻颗粒中黄芩苷的含量。方法：采用HPLC法,流动相为甲醇-0.1%磷酸（47∶53）,流速为1 ml/min,检测波长为280 nm,柱温：室温,测定方中黄芩苷含量。结果：黄芩苷在6.16~197.2μg/ml范围内呈良好线性关系（r=0.9998,n=6）,平均加样回收率为100.23%,RSD为1.53%。结论：本法快速、简便、准确、重复性好、结果可靠,适用于急麻颗粒中黄芩苷的含量测定。
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| 关 键 词： | 急麻颗粒 黄芩苷 高效液相色谱法 |
Determination the content of baicalin in Jima granules with HPLC |
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| Liu Hong,Liu Hui,Liu Binguo.Determination the content of baicalin in Jima granules with HPLC[J].Tianjin Pharmacy,2014(6):11-12. |
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| Authors: | Liu Hong Liu Hui Liu Binguo |
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| Institution: | ( No. 254 Hospital of PLA , Tianjin 300142) |
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| Abstract: | Objective： To develop a HPLC method for the determination of baicalin in Jima granules. Methods： HPLC was performed on ZORBAX SB- C18（ 4. 6 mm × 150 mm,5 μm） column with mobile phase consisted of methanol- 0. 1% phosphoric acid（ 47∶53） at 25 ℃. The flow rate was 1. 0 ml/min. The detection wavelength was 280 nm. Results： Baicalin showed a good linearity in the range of 6. 16 ~ 197. 2 μg / ml（ r = 0. 9998）,and the average recovery was 100. 58%（ RSD was 1. 57%）. Conclusion： This methods is simple,reliable and suitable for quantitative determination of baicalin in Jima granules. |
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| Keywords: | Jima granules baicalin HPLC |
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