LY294002对胃癌细胞株生长增殖及侵袭能力的影响

目的探讨LY294002（PI3K/Akt信号通路特异性阻断剂）对胃癌细胞生长增殖、凋亡和侵袭转移的影响。方法采用不同浓度LY294002处理胃癌AGS细胞,MTT法检测胃癌AGS细胞经LY294002作用后生存率的变化;流式细胞术检测细胞周期和凋亡的变化;Transwell实验和划痕实验分析细胞侵袭、转移能力的改变。结果 MTT检测结果显示,一定浓度的LY294002可抑制AGS细胞的生长,且随着浓度的增加和作用时间的延长,其抑制作用明显增强（P〈0.05）。流式细胞术显示,AGS细胞经LY294002作用48h后,细胞周期阻滞于G1期,G1期细胞比例上升,S期细胞比例下降,细胞有明显凋亡,并呈现浓度依赖性（P〈0.05）。Transwell实验和划痕实验显示,AGS细胞经LY29400作用后,其侵袭和转移能力受到抑制。结论 LY294002可抑制胃癌AGS细胞的生长增殖,且呈时间和剂量的依赖性。其机制可能是阻滞细胞周期及诱导细胞凋亡。LY294002也可抑制胃癌AGS细胞的侵袭和转移。

Effect of LY294002 on the Proliferation and Invasion of Gastric Cancer Cell Lines

Objective To investigate the effects of LY294002,a specific inhibitor of phosphatidylinositol 3-kinase,on the growth,proliferation,apoptosis,migration and invasion ofgastric cancer cell lines.Methods The growth and proliferation of AGS cells treated with different concentrations of LY294002 were measured by MTT assay.Cell cycle and apoptosis rate of AGS cells were analyzed by flow cytometry （FCM）.The ability of cells＇ invasion and migration were evaluated by would healing and Transwell invasion assay.Results After being cultured with LY294002,the growth of AGS cells was significantly inhibited in dose and time dependent manner （P0.05）.Flow cytometry displayed that LY294002 was able to raise AGS cells＇ apoptosis and induce G1 phase arrest in dose dependence（P0.05）.Would healing and Transwell invasion assay showed that the ability of cells＇ migration and invasion were inhibited.Conclusion LY294002 can significantly inhibit the growth and proliferation of AGS cells in a dose-and-time dependent manner,and its mechanisms may arrest cell cycle and induce apoptosis;LY294002 can inhibit the migration and invasion of AGS cells.