As_2O_3联合~（89）SrCl_2对人乳腺癌细胞株MCF-7细胞周期和凋亡的影响

目的探讨三氧化二砷（As2O3）联合氯化锶（89SrCl2）对人乳腺癌细胞株MCF-7细胞周期与凋亡的影响。方法采用MTT比色法检测As2O3对MCF-7细胞的增殖抑制作用,求出细胞半数抑制浓度（ID50）。选择20%ID50以下两个不同浓度的As2O3联合89Sr照射,随机设置对照组、89SrCl2照射组、As2O3处理组,As2O3＋89SrCl2联合处理组（联合组）,并于处理后24h采用流式细胞术检测各组细胞周期分布及凋亡。结果 As2O3能明显抑制MCF-7细胞增殖,给药24h的ID50为11.7μmol/L。细胞流式术检测结果表明,与89SrCl2照射组相比,联合组G2/M期细胞明显增多（P〈0.05或0.01）,早期凋亡和死亡细胞数显著增高（P〈0.05）。结论 As2O3能够促进89Sr照射诱导的MCF-7细胞周期阻滞与凋亡。

Effects of Arsenic Trioxide and Strontium-89 Chloride on the Cell Cycle and Apoptosis of Human Breast Cancer Cell line MCF-7

Objective To investigate the effects of arsenic trioxide （ As2O3） and 89SrCl2（ strontium89 chloride） co-treatment on the cell cycle and apoptosis of MCF-7 human breast cancer cell line. Methods The MTT method was applied to explored the impacts of As2O3on the proliferation of MCF-7 cell , and select the proper concentration of As2O3 to use. The cells were randomly divided into four groups： control group,As2O3treatment group,89SrCl2 irradiation group and As2O3and 89SrCl2 co-treatment group （ combining group） . The cell cycle distribution and apoptosis were analyzed by flow cytometry at 24 h after treatment. Results The results showed As2O3 could significantly inhibite the growth of MCF-7 cells and the 24 h ID50 was 11. 7μmol/L. The cells during G2 /M phase in combining group was significantly more than that in 89SrCl2 irradiation group and the death cells and cells at early stage of apoptosis in combining group predominantly increased,significantly different from that in 89Sr irradiation group （ P 0. 05 or 0. 01） . Conclusions As2O3could promote G2 arrest apoptosis of the MCF-7 cells induced by exposure to89SrCl2.