骨髓基质细胞复合聚DL-乳酸/羟基磷灰石修复兔桡骨缺损的研究

目的探讨复合兔骨髓基质细胞（BMSCs）的聚DL-乳酸/羟基磷灰石（PDLLA/HA）在修复兔桡骨缺损中的效果。方法 36只健康新西兰白兔,共72侧前肢,造成1.0cm长的桡骨缺损,随机分成A、B、C3组,A组植入BMSCs＋PDLLA/HA,B组单纯植入PDLLA/HA,C组不做任何植入,于术后2、4、8、12周时行X线、大体和组织学观察。结果 A组成骨效果优于B组,12周时已完全骨性愈合,髓腔通畅,在各骨缺损修复时期,其成骨速度、成骨量和再生髓腔结构均优于B组、C组;A组X线评分也高于B组,差异均有统计学意义（均P〈0.01）。结论 BMSCs复合PDLLA/HA具有较强的成骨能力,有望成为临床修复骨缺损的治疗方法。

PDLLA/HA Combined with Bone Marrow Stromal Cells for Repairing Radial Defects in Rabbits

Objective To study the defects of combined use of PDLLA/HA and BMSCs on repairing segmental bone defects. Methods 1. 0 cm radius defect was made in 72 forelimbs of 36 adult New Zealand white rabbits. The 72 forelimbs were randomized into 3 groups,the defects of group A were filled with BMSCs-PDLLA/HA,the defects of group B were treated with PDLLA/HA,group C was untreated（ empty） to serve as the control group. The healing response was evaluated macroscopically,histologically and radiographically at two,four,eight,and twelve weeks after implantation. Results Group A exhibited the strongest defect-repairing ability ,and obvious healing of could be seen 12 weeks postoperatively. At every stage,in group A there were better results than those in group B in terms of quantity and quality of new bone growth. The score of X-ray in the implants loaded with BMSCs was higher than that in the cellfree implants（ P 0. 01） . Conclusions Since the BMSCs-PDLLA/HA can repair segmental bone defects,it can be used in the clinical treatment of bone defects.