降植烷诱导C57BL/J6狼疮性肾病鼠模型的建立和生物学鉴定

目的构建C57BL/J6狼疮性肾病（LN）鼠模型,探讨降植烷在其发病机制中的生物学作用。方法 C57BL/J6小鼠随机分为两组,实验组予一次性腹腔注射降植烷0.5ml,对照组予一次性腹腔注射生理盐水0.5ml。注射后第5、10天分别检测脾脏中吞噬细胞、树突状细胞及B细胞表面分子的变化情况;每月定期检测血清中抗核抗体（ANA）、抗双链DNA抗体（dsDNA）及尿蛋白,7个月后观测肾脏HE染色和免疫复合物（IC）的沉积情况。结果小鼠腹腔注射降植烷第5、10天后,脾脏中的吞噬细胞、树突状细胞不同程度活化（P〈0.05）,B细胞表面CD21、CD86、MHC-Ⅱ等分子表达也不同程度上调（P〈0.05）;4个月后血清ANA、dsDNA有不同程度的表达（P〈0.05）;7个月后,78%的小鼠出现蛋白尿（≥＋）,肾脏HE切片显示肾脏出现肾小球肿胀,淋巴细胞浸润等典型的肾病改变,IC沉积。而对照组中血清ANA、dsDNA阴性,未见蛋白尿,肾脏组织未见病理改变。结论降植烷成功地诱导了C57BL/J6LN鼠模型。

Developing and Charactering C57BL/J6 Murine Lupus Model Induced by Pristane

Objective To explore the pathogenic mechanism of a murine model of systemic lupus erythematosus（SLE） in C57BL/J6 induced by Pristane.Methods C57BL/J6 mice were divided into two groups randomly.Animals in the experimental group was injected with 0.5 ml Pristane by intraperitoneal injection while in the control group with 0.5 ml normal saline（NS）.The activation of macrophage and dendritic cell in spleens and markers on B cells were measured at day 5 and 10 after injection,respectively.Antinuclear antibodies（ANA） and anti-double strand DNA antibodies（dsDNA） were checked accordingly.Seven months after injection,all mice were killed and serum were detected monthly after injection meanwhile proteinuria kidneys were slided and stained with HE or FITC-labeled IgG to observe the evidence of glomerulonephritis histopathologically.Results After Pristane injection,the populations of macrophage and dendritic cell were increased significantly compared to the control ones（P 0.05）.The expressions of CD21,CD86,MHC-Ⅱ on B cells were also increased（P 0.05）.Autoantibody ANA and dsDNA appeared in serum as early as 4 months after Pristane injection（P 0.05）.Seven months later,the protein concentration of uria in most experimantal mice was ≥ ＋.Both light microscopy and imunoflorescence of kidney sections indicated typical evidence of glomerulonephritis.In the control group,proteinuria wasn＇t detected under the same experiamental condition.Meanwhile,there was no evidence of glomerulonephritis in the control group.Conclusion Murine model of SLE in C57BL/J6 with Pristane is established successfully.