青蒿素对人宫颈癌细胞的毒性和放射增敏研究

目的探讨青蒿素对宫颈癌细胞系HeLa的毒性和放射增敏作用。方法采用MTT法检测青蒿素对HeLa细胞的毒性,测出青蒿素的最适作用浓度和作用时间。用MTT法检测青蒿素对HeLa细胞放射增敏的影响,用多靶单击方程拟合HeLa细胞的剂量存活曲线求出辐射增敏比,评价增敏效果。用流式细胞仪检测细胞周期。结果青蒿素与HeLa细胞作用24h的IC50为600.19nmol/ml,作用48h的IC50为160.71nmol/ml。青蒿素对HeLa细胞的辐射增敏比（SER）为1.17。单放组和增敏组放疗后24h的周期变化,不同照射剂量的增敏组G2/M期比例下降。结论青蒿素对宫颈癌细胞系HeLa有毒性作用,且有剂量依赖性和时间依赖性。青蒿素对HeLa细胞有放射增敏性,青蒿素能抑制电离辐射诱导的G2期阻滞。

Cytotoxic Effect and Radiation Enhancement of Artemisinin in Uterine Cervical Carcinoma Cell Line HeLa

Objective To investigate cytotoxic and radiosensitizing effect of Artemisinin on cervical carcinoma cell line HeLa.Methods In order to measure the optimized effective time,cytotoxic effect of Artemisinin on HeLa cell line was investigated with MTT assay.The radiosensitization effect of different doses and different treatment duration of Artemisinin on HeLa cell line were evaluated by MTT test,the SER is 1.17 and radiosensitizing effect was measured with multi-target single hit model through SER of HeLa cell.Cell cycles in different groups were calculated by flow cytometry.Results The 50% inhibition concentration of Artemisinin interacted with HeLa cells for 24 h is 600.19 nmol/ml,and for 48 h is 160.71nmol/ml.The HeLa cells’surival ratio is 93.51%、91.87%、and 87.28% after adding Atemisinin of 110.69nmol/ml and 1Gy radiation exposure.There are three groups：the chemotherapy only group,the radiotherapy only group and the combination group.The result of the cell cycles showed that cells in G2/M period decreased in the combination group.Conclusion Artemisinin has radiosensitization effect on cervical carcinoma HeLa cells,whichshows dose and time dependent.Artemisinin can inhibit the G2/M block by ionizing radiation.