| 连云港地区汉族人群15个STR基因座的遗传多态性 |
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| 引用本文： | 王军,邢锦山.连云港地区汉族人群15个STR基因座的遗传多态性[J].苏州大学学报(自然科学版),2010,30(2):333-335. |
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| 作者姓名： | 王军 邢锦山 |
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| 作者单位： | 王军,WANG Jun(连云港市公安司法鉴定中心,江苏连云港222003;南京市浦口区公安司法鉴定中心,江苏南京211800);邢锦山,XING Jin-shan(连云港市公安司法鉴定中心,江苏连云港,222003) |
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| 摘 要： | 目的调查连云港地区汉族无关个体的15个STR基因座（D8S1179、D21S11、CSF1PO、D3S1358、D7S820、TH01、D13S317、D2S1338、D18S51、D16S539、TPOX、vWA、D19S433、D5S818、FGA）多态性,研究其在法医学检验中的应用价值。方法用AmpFlSTR IdentifilerTM五色荧光标记系统对新鲜血样进行15个基因座的复合扩增,用ABI3130XL全自动测序仪对扩增产物进行检测,用GeneMapper软件进行基因分型。结果 15个基因座累积个人识别能力值为0.9999999999,累积非父排除率达99.999%,家系调查符合孟德尔遗传规律。结论含有15个STR基因座的五色荧光标记复合扩增系统适合用于个体识别和亲子鉴定。
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| 关 键 词： | 法医物证学 STR基因座 PCR 遗传多态性 |
Genetic Polymorphism of 15 STR Loci Mutiplexing in Lianyungang Han Population |
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| WANG Jun,XING Jin-shan.Genetic Polymorphism of 15 STR Loci Mutiplexing in Lianyungang Han Population[J].Suzhou University Journal of Medical Science,2010,30(2):333-335. |
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| Authors: | WANG Jun XING Jin-shan |
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| Institution: | 1.Lianyungang Criminal Science ＆ Technology Institute,Jiangsu Lianyungang 222003,China;2 Nanjing Pukou District Criminal Science ＆ Technology Institute,Jiangsu Nanjing 211800,China) |
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| Abstract: | Objective The genetic studies of 15 short tandem repeats（STRs） loci in AmpFISTR IdentifilerTM multiplex amplification systems was carried out in Chinese Han population in Lianyungang.Methods DNA samples from 500 unrelated individuals were screened.The 15 loci were D8S1179、D21S11、CSF1PO、D3S1358、D7S820、TH01、D13S317、D2S1338、D18S51、D16S539、TPOX、vWA、D19S433、D5S818 and FGA.The PCR products were analyzed and genotyped by ABI 3130XL Sequencer.Results The combined power of discrimination （DP） was 0.999 999 999 9 and the combined paternity of exclusion（PE） was 99.999%.These 15 loci met the Hardy-Weinberg expectations.Conclusion The AmpFISTR IdentifilerTM multiplex amplification systems was very useful in forensic case investigation. |
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| Keywords: | PCR |
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