干扰素调节因子-5基因多态性与类风湿关节炎的相关性

目的探讨干扰素调节因子-5（IRF-5）基因rs2004640位点基因多态性与河北汉族人群类风湿关节炎（RA）的相关性。方法应用聚合酶链反应-连接酶检测反应（PCR-LDR）法检测河北汉族人群136例RA患者和152名正常对照者IRF-5基因rs2004640位点多态性。应用ELISA法对RA患者进行抗环瓜氨酸抗原肽（CCP）抗体检测。结果 IRF-5基因rs2004640位点T等位基因频率在RA组明显高于对照组（28.1%vs20.4%）,两组差异有统计学意义（χ2=4.257,P〈0.05,OR=1.522,95%CI：1.038～2.231）。进一步将RA组按抗环瓜氨酸抗原肽（CCP）抗体阴性、阳性分层发现,抗CCP抗体阴性RA患者与对照组人群比较,T等位基因频率的差异有高度统计学意义（χ2=6.803,P〈0.01,OR=1.980,95%CI：1.209～3.242）。结论 IRF-5 rs2004640基因多态性与抗CCP抗体阴性RA患者发病存在相关性。

Association of Interferon Regulatory Factor 5 Gene Polymorphism with Rheumatoid Arthritis

Objective To investigate the association between the polymorphism of interferon regulatory factor-5（IRF-5） rs2004640 site and rheumatoid arthritis（RA） in Han population in Hebei.Me-thods IRF-5 rs2004640 site was genotyped by polymerase chain reaction-ligase detection reaction（PCR-LDR） in 139 patients and 152 controls.All patients were tested for the presence of anti-cyclic citrullinated peptides（anti-CCP） by ELISA.Results The T alleles frequencies of IRF-5 rs2004640 in cases were higher than that in the controls（28.1% vs 20.4%）,the differences was statistic significant（χ2=4.257,P0.05,OR=1.522,95% CI：1.038~2.231）.There were also significant differences in T allele frequency between the anti-CCP negative RA patients and the controls（χ2=6.803,P0.01,OR=1.980,95% CI：1.209~3.242）.Conclusion This study suggests that the IRF-5 gene polymorphism is associated with the anti-CCP negative RA in Chinese Han population in Hebei province.