不同环境对湖北贝母总生物碱含量与指纹图谱的影响

［摘要］目的确定湖北贝母生长的最佳生长年限、规格及施肥方案，为湖北贝母GAP研究提供基础资料。方法对不同生长年限、规格及施用不同肥料的湖北贝母采用酸性染料比色法，在415 nm波长下测定紫外吸收，比较其总生物碱含量。采用高效液相色谱 蒸发光散射（HPLC ELSD）检测法，梯度洗脱得到指纹图谱，比较色谱图及单体生物碱的含量。 结果一年生大粒的湖北贝母总生物碱含量最高，为0.526%（P＜0.05），一年生小粒样品湖贝甲素含量最高，为0.069%，浙贝甲素以一年生大粒的贝母含量最高，为0.065%（P＜0.05）。不施肥组的湖北贝母总生物碱、湖贝甲素、浙贝甲素含量均为最高，分别是0.551%，0.060%，0.042%。结论在其他条件相同的情况下，一年生大粒且不施肥组的湖北贝母品质要优于其他组的湖北贝母。

Effects of Different Habitas on Total Alkaloids and HPLC Fingerprints of Fritillaria Hupehensis

Objective To determine the optimum plant age,size and manure scheme of Fritillaria hupehensis,and supply the fundamental data for GAP.Methods Acid dye colorimetry was used to determine total alkaloids in Fritillaria hupehensis at 415 nm.The fingerprints are performed by HPLC-ELSD,with gradient elution.Results The contents of total alkaloids in the group of one-year old and big size are higher(0.526%)than that in other groups and peimine is higher(0.065%)in the group of one-year old and big size.Hupehenine in the one-year group of small size is the highest,0.069%.The contents of total alkaloids,hupehenine and peimine(respectively 0.551%,0.060% and 0.042%)in the group without fertilizer are higher than those in other groups.Conclusion Under the other same conditions,the quality of one-year and big size Fritillaria hupehensis and without fertilizer is better than that of other Fritillaria hupehensis.