| 基于生物信息学分析方法设计流感病毒A的通用寡核苷酸探针 |
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| 引用本文： | 王兴友,陈杭薇,李兵. 基于生物信息学分析方法设计流感病毒A的通用寡核苷酸探针[J]. 华北国防医药, 2006, 18(5): 310-312 |
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| 作者姓名： | 王兴友 陈杭薇 李兵 |
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| 作者单位： | 北京军区总医院呼吸内科,北京100700 |
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| 基金项目： | 全军"十一五"科技攻关项目 |
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| 摘 要： | 目的:设计流感病毒A诊断芯片的寡核苷酸(Oligo)探针.方法:利用BLAST软件对流感病毒A的基因序列进行比对,得到有意义的特异序列;用生物学软件Array Designer4.2设计特异性高、Tm值相近、长度均一的Oligo探针.结果:获得10条30 mer的Oligo探针.结论:利用BLAST软件和生物学软件Array Designer4.2设计流感病毒A诊断芯片的探针,可为后期打印成基因芯片,用于流感病毒A的检测打下基础.
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| 关 键 词： | 流感病毒A型 生物芯片 Oligo探针 BLAST软件 |
| 文章编号： | 1009-0878(2006)05-0310-02 |
| 收稿时间： | 2006-06-01 |
| 修稿时间： | 2006-08-24 |
The Design of Oligonucleotide Probe in Influenza Virus A Based on Bioinformatics |
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| WANG Xing-You,CHEN Hang-wei,LI Bing. The Design of Oligonucleotide Probe in Influenza Virus A Based on Bioinformatics[J]. Medical Journal of Beijing Military Region, 2006, 18(5): 310-312 |
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| Authors: | WANG Xing-You CHEN Hang-wei LI Bing |
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| Affiliation: | Department of Respiration, General Hospital of Beijing Military Area, Beijing 100700 ,China |
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| Abstract: | Objective:To design oligonucleotide probes for microarray detection of Influenza virus Type A.Methods:By analyzing the gene of Influenza Virus A with BLAST program,a group of specific sequences for the candidate probes were acquired.Array Designer4.2 software was applied to analyze the candidates to select the probes with high specificity,identical length and similar melting temperature(Tm).Results:10 oligonucleotide probes were designed.Conclusion:Oligonucleotide probes can be designed with BLAST program and Array Designer4.2 software,and become a foundation for depositing on gene chips as the basis of the microarray for Influenza Virus A detection. |
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| Keywords: | Influenza Virus A Biochip Oligonucleotide probe Basic local alignment search tool |
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