精子上游器的研制及其实验效果 |
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引用本文: | 张鹏李军涛,路西兰等. 精子上游器的研制及其实验效果[J]. 中国性科学, 2014, 0(5): 25-27 |
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作者姓名: | 张鹏李军涛 路西兰等 |
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作者单位: | [1]潍坊医学院研究生院,山东潍坊261053; [2]山东大学附属济南市中心医院生殖医学科,济南250013; [3]山东省妇产医院生殖医学科,济南250001 |
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摘 要: | 目的:研制一种新型精子优选器皿—精子上游器(发明专利号:ZL 2008 10016424.1),并检测其实验效果。方法:采用精液样本30份,每份精液样本平分为两份,分别用精子上游器(实验组)和传统的四试管上游法(对照组)上游处理,然后分析处理后精子的浓度和活力及前向运动精子数。结果:实验组精液a级、前向运动精子率和前向运动精子数均高于对照组,差异有统计学意义(P值均〈0.001)。实验组精子浓度低于对照组,差异有统计学意义(P〈0.05)。结论:本项目研制的精子上游器使用方便、易于操作,满足辅助生殖技术的临床要求,值得推广应用。
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关 键 词: | 精子上游器 精子浓度 精子活力 |
Development of sperm swim-up device and its laboratory efficacy |
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Affiliation: | ZHANG Peng, LI Juntao, LU Xilan, XU Xiangling, ZHANG Haiyan, ZHANG Jianmin, WANG Hengcai2A. 1. Graduate School of Weifang Medical U- niversity, Weifang 261053, China; 2. Department of Reproductive Medicine, Jiuan Central Hospital Affiliated to Shandong University, Jinan 250013, China; 3. Department of Reproductive Medicine, Maternity Hospital of Shan- dong , Jinan 250001, China |
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Abstract: | Objectives: To develop a new type of spermatozoa sorting instrument-sperm swim-up device-( whose invention and patent No. is ZL 2008 10016424. 1) and test its experimental effect. Method: 30 semen samples were selected,each being divided into two equal parts and processed by sperm swim- up device( case group) or traditional four- tube- swim- up method( control group) respectively. After that we contrasted and analyzed the sperm concentration,motility and forward movement sperm count of the 2 groups. Results: The percentages of grade a,forward movement spermatozoon and forward movement sperm count in experimental group were all higher than those in the control group; the differences were all statistically significant( P〈0. 001); The sperm concentration of experimental group was lower than that in the control group,with a statistically significant difference( P〈0. 05). Conclusion: The sperm swim- up device developed in the project is easy to use and operate,which meets the clinical requirement of assisted reproductive technology and is worthy of popularization and application. |
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Keywords: | Sperm swim-up device Sperm concentration Sperm motility |
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