Alterations of in vitro interleukin 1 and 2 in diabetic children |
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Authors: | R Lorini D Montagna A Lanfranchi L Cortona C Livieri D Larizza G d'Annunzio F Severi |
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Institution: | (1) Department of Paediatrics, I and II Chair, University of Pavia, Pavia, Italy;(2) Clinica Pediatrica, Policlinico San Matteo, P. le Golgi, I-27100 Pavia, Italy |
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Abstract: | We studied interleukin 1 (IL-1) and interleukin 2 (IL-2) production in unstimulated and stimulated cultures from 27 young diabetic patients and 21 age-matched healthy subjects. In unstimulated cultures monocytes from newly diagnosed patients produced significantly higher levels of IL-1 than controls. In lipopolysaccharide (LPS)-stimulated cultures, IL-1 production in patients with fresh and long-standing diabetes was no different from that of controls. IL-2 production was low or absent in unstimulated cultures from insulin-dependent diabetes mellitus (IDDM) patients and controls. In phytohaemagglutinin (PHA)-stimulated cultures both patient groups produced significantly less IL-2 than controls. No correlation was observed between IL-1, IL-2 production and HbA1 levels or the presence of HLA-DR3 or DR4. Our data on spontaneous IL-1 production support the hypothesis that monocytes from some newly diagnosed IDDM patients may circulate in a preactivated state. The low levels of IL-2 might be explained by an abnormal consumption or by the presence of increased soluble IL-2 receptor levels or by a serum factor which interferes with IL-2 production. Alternatively, it may be a genetically determined trait.Abbreviations AU
arbitrary units
- CTLL
cytotoxic T lymphocyte line
- ICA
islet cell antibodies
- IDDM
insulin-dependent diabetes mellitus
- IL-1
interleukin 1
- IL-2
interleukin 2
- LPS
lipopolysaccharide
- PHA
phytohaemagglutinin
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3H-TdR
tritiated thymidine |
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Keywords: | Insulin-dependent diabetes mellitus Interleukin-1 and 2 Immunoregulation |
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