纳米磁性靶向药囊对人肝癌裸鼠移植瘤bcl-2/bax蛋白表达的影响

目的观察纳米磁性5-Fu药囊靶向治疗人肝癌裸鼠移植瘤对肿瘤细胞凋亡bcl-2及bax蛋白表达的影响。方法透射电镜观察各组移植瘤形态学特征，免疫组化SABC法测定bcl-2和bax蛋白表达水平。结果电镜观察可见B组（5-Fu）和D组（纳米磁性5-Fu药囊加内磁场）肿瘤组织大量癌细胞凋亡。B和D组bcl-2蛋白表达水平低于A组（生理盐水对照）、C组（纳米磁性5-Fu药囊）和E组（纳米磁小体加内磁场）（P〈0．01），D组bcl-2蛋白表达水平低于B组（P〈0．01）；B组和D组bax蛋白表达水平高于其他三组（P〈0．01），D组bax蛋白表达水平高于B组（P〈0．01）。结论纳米磁性5-Fu药囊靶向治疗能够降低bcl-2蛋白表达，增加bax蛋白表达，促进人肝癌裸鼠移植瘤细胞凋亡，提高5-Fu抗肿瘤作用。

Effect of targeted magnetic nanoparticles containing 5-Fu on expression of bcl-2/bax protein in transplanted liver cancer in nude mice

Objective] To study the effect of targeted magnetic nanoparticles containing 5-Fu on expression of bcl-2/bax protein in transplanted liver cancer in nude mice.Methods] Morphological characteristics of transplanted tumors were studied by electron microscope.The expression of bcl-2/bax protein was detected by immunohistochemistry(SABC).Results] The phenomenon of apoptosis was observed in group B(5-Fu)and group D(magnetic nanoparticles containing 5-Fu and magnetic field built inside tumor tissue)by electron microscope.The expression of bcl-2 was markedly decreased but that of bax markedly increased in group B and group D than in group A(NS),group C(magnetic nanoparticles containing 5-Fu)and group E(magnetic nanoparticles without 5-Fu and magnetic field built inside tumor tissue)(P <0.01).The expression of bcl-2 was markedly decreased but that of bax markedly increased in group D than in group B(P<0.01).Conclusion] The targeted therapy of magnetic nanoparticles containing 5-Fu can inhibit the growth of hepatocellular carcinoma and up-regulate apoptosis of the liver cancer cells by decrease the expression of bcl-2 gene,increase the expression of bax gene.