| cyclin G2荧光蛋白重组质粒的构建及其对宫颈癌HeLa细胞的作用 |
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| 引用本文： | 魏娜,郑洪,汤英姿. cyclin G2荧光蛋白重组质粒的构建及其对宫颈癌HeLa细胞的作用[J]. 贵州医药, 2011, 35(8): 675-679. DOI: 10.3969/j.ISSN.1000-744X.2011.08.001 |
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| 作者姓名： | 魏娜 郑洪 汤英姿 |
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| 作者单位： | 1. 遵义医学院组胚教研室,563003
2. 遵义医学院附属医院病理科,563003 |
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| 基金项目： | 贵州省科学技术基金资助项目[黔科合J字(2007)2128号] |
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| 摘 要： | 目的 构建包含人cyclin G2基因的荧光蛋白重组质粒,探讨cyclin G2对宫颈癌HeLa细胞凋亡的影响.方法 运用RT-PCR和基因克隆技术构建pDsRed2-Cyclin G2重组质拉;脂质体法转染重组质粒至HeLa细胞中,荧光显微镜下观察其蛋白的表达和定位;流式细胞术检测HeLa细胞的凋亡率.结果 成功地构...
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| 关 键 词： | Cyclin G2 重组质粗 宫颈癌细胞系(HeLa) 凋亡 |
Construction of fluorescent protein recombinant plasmid with cyclin G2 and its function in HeLa cells |
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| Wei Na,Zheng Hong,Tang Yingzi. Construction of fluorescent protein recombinant plasmid with cyclin G2 and its function in HeLa cells[J]. Guizhou Medical Journal, 2011, 35(8): 675-679. DOI: 10.3969/j.ISSN.1000-744X.2011.08.001 |
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| Authors: | Wei Na Zheng Hong Tang Yingzi |
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| Affiliation: | Wei Na,Zheng Hong,Tang Yingzi.Department of histology and embryology,Zunyi Medical College,Zunyi 563003,China. Department of Pathology,China |
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| Abstract: | Objective To construct human cyclin G2 gene eukaryotic vector and study the effect on HeLa cells growth in vitro. Methods The pDsRed2-cyclin G2 fusion protein expression vectors were constructed by RT-PCR and gene cloning techniques.Then it was transferred into HeLa cells by cation lipofectin reagent.Expression,intracellular localization and function of this fusion protein in HeLa cells were observed by fluorescence microscope after transfection.The apoptosis were analyzed by FCM after 48 hours. Results cyc... |
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| Keywords: | cyclin G2 Recombinant plasmid Uterine cervix cancer cells lines(HeLa) Apoptosis |
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