三七总皂甙对兔肺挫伤治疗作用的实验研究

目的 采用重物自由落体复制兔肺挫伤模型,通过检测炎症因子白介素-1β(interleukin-1β,IL-lβ),氧化应激指标脂质过氧化产物丙二醛 (malondialdehyde,MDA)及髓过氧化物酶(myeloperoxidase,MPO)水平,并结合肺组织湿干比重及病理变化,探讨三七总皂甙(panax noto...

Experimental study on effects of Panax Notoginseng Saponins(PNS) on pulmonary contusion in rabbits.

Objective The study was to explore the effect and the possible mechanism of PNS treating pulmonary contusion induced by heavy free-railers on rabbits by measuring IL-1β, MDA and MPO combined with wet to dry proportion and pathological changes of lung tissues, providing the experimental and theoretical evidence for the clinical using of PNS. Methods 24 healthy adult rabbits were randomly divided into control group which were contusion group and treatment group （n=8）. Control and contusion groups were pumped into the saline （5 mL/kg） after moulding five minutes by the right ear margin vein. The treatment group were pumped the injectable venous thrombus tong （freeze-dried 150 mg/kg）, all were pumped in within ten minutes. The blood samples were collected from left femoral vein 0.5 h before impacting and 0.5 h, 2 h, 4 h, 6 h, after impacting. At the end of the experiment,the serum was used to detected IL-1β levels, small lung tissue were collected to measured MDA and MPO content, W/D, pathological changes of lung tissue were observed in the light microscope. Results Serum IL-1L3 level in contusion group increased significantly compared with control group （P~0. 01）. Serum IL-1β level in treatment group showed slow declining, compared with contu- sion group （P〈0.01）. The MDA content and W/D of lung tissue in contusion group was higher than the control group （P〈0. 05） ,the MPO content was significantly higher than the control group （P〈0.01）. The above all were decreased after PNS intervention （P〈0.05）, but higher than control group （P〈0.05）. Pathological studies showed increased lung contusion volume, scattered hatches in the congestion, sliced ？ red solid-like change. Microscope showed pulmonary interstitial edema, exudation, hemorrhage, granulocyte infiltration and destruction of alveolar structure. Pathological injury was significantly reduced in treatment group compared with contusion group. Conclusion PNS can re duce lung tissue damage induced by inflammation and oxidative stress by lowering serum IL-1β levels and MDA, MPO content of lung tissue; PNS improves the pulmonary hemorrhage, edema and neutrophil infiltration. PNS has better protection function for the rabbit acute pulmonary contusion and pro vides a new approach for the treatment of acute pulmonary contusion.