嗅鞘细胞条件培养液对PC12细胞神经丝蛋白?突触蛋白?突触素蛋白表达的影响

目的:观察嗅鞘细胞(olfactory ensheathing cells,OECs)条件培养液对PC12细胞神经丝蛋白(neurofilament protein,NF)、突触蛋白(synapsin)和突触素蛋白(synaptophysin)表达的影响,探讨嗅鞘细胞条件培养液促神经细胞分化生长的作用机制。方法:原代培养并纯化OECs,收集培养上清制备成嗅鞘细胞培养液(OEC culture medium,OECCM),与PC12细胞培养24、48、72h,观察细胞形态学变化;通过免疫荧光法检测PC12细胞NF的表达及分布;蛋白质印迹法检测NF、突触蛋白和突触素的相对含量。结果:OECCM培养的PC12细胞长有突起,并随着培养时间的延长,细胞形态酷似神经元;免疫荧光染色显示NF起初在核周分布,OECCM作用后NF的分布范围逐渐增加,其分布面积与β-tubulin分布面积的比值显著增高(P<0.05)。蛋白质印迹法显示OECCM组NF、突触蛋白(synapsin)和突触素蛋白(synaptophysin)的表达水平明显高于对照组(P<0.05)。结论:嗅鞘细胞能分泌一些促PC12细胞分化的因子,这些因子能促进P...

Effect of olfactory ensheathing cell conditioned medium on NF,synapsin and synaptophysin expression in PC12 cells

Objective:To observe the effect of olfactory ensheathing cells (OECs) conditioned medium on NF,synapsin and synaptophysin expression in PC12 cells,and investigate the mechanism of olfactory ensheathing cell conditioned medium to promote growth of nerve cell differentiation. Methods:Primarily cultured OECs were prepared and purified,and the purified cell conditioned medium was collected into the olfactory ensheathing cell culture medium,then used to culture PC12 cells in 24 h,48 h,72 h. In vitro the morpholo...