自体外周血干细胞/脱钙骨复合移植治疗兔桡骨缺损的实验研究

目的 观察自体外周血干细胞(Autologous Pexipheral Blood Stem Cell—APBSC)/脱钙骨(Decalcified Bone Matrix-DBM)复合移植治疗骨缺损的疗效。方法 36只家兔桡骨做成1cm骨缺损,随机分为DBM组自体红骨髓(Autologous red bone marrow—ARBM)/DBM组APBSC/DBM组,每组12只。分别进行X线观察、生物力学检查和组织学观察的对比研究。结果 术后第2、4、8、14周APBSE/DBM组X线观察改进的Gary评分和光镜观察均优于DBM组,与ARBM/DBM组相似,术后第14周APBSC/DBM组整骨破坏载荷和骨缺损修复,形态学评分也优于DBM组,与ARBM/DBM组比较,前者没有显著性差异,后者有显著性差异。结论 APBSC/DBM复合移植治疗骨缺损的疗效优于单纯DBM移植,与ARBM/DBM组相似。结合临床我们认为具有良好的应用前景。

An Experimental Study on the Repairing of Rabbit Radial Defects by Composite Grafting of Autologous Peripheral Blood Stem Cell and Homogenous Decalcified Bone Matrix

Objective To observe the effect of repairing segmental bony defects with the composite grafting of autologous peripheral blood stem cell(APBSC) and decalcified bone matrix(DBM) . Methods 36 rabbits were divided into 3 groups randomly. On the average, 12 rabbits in each group - DBM; autologous red bone marrow(ARBM/DBM);and APBSC/DBM. A segmental bone defectdcm in the length)was created at two radii in each rabbit. The curative effect of segmental bone defects was assessed by serial radiographic, histologic studies and biomechanical test respectively. Results APBSC/DBM was superior to DBM, and similar to ARBM/DBM in X-ray, modified Gary' s radiographic scoring and microscope test in the second, fourth, eighth, and fourteenth week after operation; APBSC/DBM was also superior to DBM in the load broken of intact bone and the scoring histomorphology in bone defects repairing in the fourteenth week after operatrion. But compared with ARBM/DBM, there was not a significant difference in the load broken of intact bone, and was in the scoring histomorphology in bone defects repairing. Conclusions The composite grafting of APBSC/DBM is superior to DBM alone, and similar to the composite grafting of ARBM/DBM in the curative effect of bone defects reparing. From the view of clinical practice, the methods may show a better prospect.