| 高效液相色谱法同时测定复方柳安咖注射液中3组分含量 |
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| 引用本文： | 辛俊衡,汪锌华.高效液相色谱法同时测定复方柳安咖注射液中3组分含量[J].中国药业,2008,17(18):39-40. |
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| 作者姓名： | 辛俊衡 汪锌华 |
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| 作者单位： | 广西壮族自治区梧州食品药品检验所,广西,梧州,543002 |
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| 摘 要： | 目的用高效液相色谱（HPLC）法测定复方柳安咖注射液中水杨酸钠、安替比林和咖啡因含量。方法采用Kromasil C18柱（250mm×4.6mm，5μm），以乙腈-0．1％磷酸二氢钾溶液（15：85）为流动相，流速为1.0mL／min，检测波长为272nm。结果水杨酸钠、安替比林和咖啡因进样量分别在0．7000～2、100Ixg（r=0.9999），0.2000--0.6000μg（r=0．9999）和0.1000—0．3000μg（r=0．9999）范围内与峰面积线性关系良好，平均回收率分别为99．1％，99.4％，99.7％，RSD分别为0．6％，0．5％，0．4％（n=9）。结论HPLC法可用于同时测定复方柳安咖注射液中水杨酸钠、安替比林和咖啡因的含量。
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| 关 键 词： | 高效液相色谱法 水杨酸钠 安替比林 咖啡因 含量测定 |
Simultaneous Determination of Three Components in Compound Sodium Salicylate, Antipyrineand Caffeine Injection by HPLC |
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| Xin Junheng,Wang Xinhua.Simultaneous Determination of Three Components in Compound Sodium Salicylate, Antipyrineand Caffeine Injection by HPLC[J].China Pharmaceuticals,2008,17(18):39-40. |
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| Authors: | Xin Junheng Wang Xinhua |
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| Institution: | (Guangxi Wuzhou Institute for Food and Drug Control, Wuzhou, Guangxi, China 543002) |
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| Abstract: | Objective To establish the HPLC method for the determination of three components in compound sodium salicylate, antipyrine and caffeine injection, Methods The Kromasil C,s column（250 mm×4.6 mm, 5μm） was used with acetonitrile-0.1% potassium dihydrogen phosphate solution （15：85） as the mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 272 nm. Results The linear ranges of sodium salieylate, antipyrine and caffeine were 0. 700 0 -2. 100μg（ r=0. 999 9）, 0. 200 0 -0.600 0μg（ r =0. 999 9）and 0, 1000-0.3000μg（r=0.9999）,The average recovery rates were 99. 1%,99.4% and 99.7%,with RSD 0.6%,0.5%,0.4%（n=9）. Conclusion The method can be used for the determination of three components in compound sodium salicylate, antipyrine and caffeine injection. |
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| Keywords: | HPLC sodium salicylate antipyrine caffeine content determination |
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