拟除虫菊酯对大鼠黑质纹状体系统内酪氨酸羟化酶的影响

目的　探讨拟除虫菊酯对大鼠黑质纹状体多巴胺能系统中酪氨酸羟化酶 (TH)的影响。方法　采用不同剂量的溴氰菊酯 (DM)、氯菊酯 (PM)对雄性SD大鼠经口灌胃给药后 ,高效液相色谱 -荧光检测法(HPLC FD)分别检测其黑质、纹状体内多巴胺 (DA)及其主要代谢产物多巴克 (DOPAC)和高香草酸 (HVA)含量 ;HPLC FD方法检测TH活性 ;免疫组化以及RT PCR技术分别检测各组黑质、纹状体内的TH蛋白量及mRNA量。结果　给药各组大鼠纹状体中DA含量都有不同程度的下降 ,且 1 1 0LD50 DM组与对照组相比差异有显著性 ,DOPAC和DA更新率与对照组相比变化不大 ,差异无显著性 ;DM组黑质和纹状体中TH酶活性与mRNA水平与对照组相比 ,均有不同程度的下降 ,且差异有显著性 (P <0 0 5 ,P <0 0 1 ) ;1 1 0LD50 DM组纹状体内的TH蛋白水平与对照组相比差异有显著性 (P <0 0 1 )。结论　DM暴露可以引起大鼠黑质纹状体系统中TH活性、mRNA水平以及蛋白量下降

Effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats

Objective To study the effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats. Methods Different doses of permethrin and deltamethrin in corn oil were administered to rats by gavage for ten days, then PLC Fluorescence detection was used to analyzed the contents of dopamine(DA), DOPAC and HVA in the Substantial Nigra and Striatum. The activities of TH were also detected by HPLC Fluorescence detection. TH mRNA or TH protein levels were measured by RT PCR and immunohistochemistry method. Results Contents of DA in Striatum in 1/10LD 50 DM group have a significant change, the contents of DOPAC and the turnover rate of DA all increased( P <0 05). but DA, DOPAC and (DOPAC HVA)/DA did not have any significant change in Substantial Nigra. The activities and mRNA level of TH had obvious decrease in two DM groups, the TH protein levels also significantly decreased in 1/10LD 50 DM group. Conclusion Deltamethrin could decrease the activities, mRNA and protein levels in nigraostriatum of rats.