| 高效液相色谱法测定栀子及其炮制品中熊果酸的含量 |
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| 引用本文： | 梁迎.高效液相色谱法测定栀子及其炮制品中熊果酸的含量[J].现代食品与药品杂志,2003,13(3):2-3. |
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| 作者姓名： | 梁迎 |
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| 作者单位： | 广东燕岭医院 广州 |
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| 摘 要： | 目的 建立栀子及其炮制品中熊果酸的测定法,考察栀子炮制前后熊果酸的含量变化。方法 采用高效液相色谱法,色谱柱为Hypersil C_(18)5μm(4.6mm×250mm),流动相为0.02mol/L NaH_2PO_4(pH2.6):甲醇(16:84),检测波长220mm。结果 在23～135μg/ml范围内,呈良好的线性关系,平均加样回收率和RSD分别为97.81％、1.51％。栀子炮制后熊果酸含量下降。结论 该方法可用于栀子及炮制品的质量控制,炮制可导致栀子中熊果酸的含量下降。
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| 关 键 词： | 栀子 熊果酸 高效液相色谱法 |
| 文章编号： | 1007-9939(2003)03-0002-02 |
| 修稿时间： | 2003年2月2日 |
Determination of ursolic acid in Fructus Gardenies and its prepared medical materials by HPLC |
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| Liang Ying.Determination of ursolic acid in Fructus Gardenies and its prepared medical materials by HPLC[J].JOurnal of Modern Food and Pharmaceuticals,2003,13(3):2-3. |
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| Authors: | Liang Ying |
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| Abstract: | Objective To establish a HPLC method for the determination of ursolic acid in Fructus Gardeniae and study the quantitative change after prepared. Methods HPLC method was used. The column was Hypersil C18 5 um(4. 6 mm x 250 mm). The mobil phase was 0.02 mol/L NaH2PO4 (pH2. 6) :methanol(16: 84). The detective wavelength was 220 mm. Results The average recovery was 97,81% ,RSD 1.51%. Conclusion The method was sample and accurate. It can be used for the quality control of Fructus Gardeniae. Roast could lead to quantitative decrease of ursolic acid in Fructus Gardeniae. |
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| Keywords: | Fructus Gardeniae ursolic acid HPLC |
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