大鼠前脑缺血再灌注不同时间神经元损伤情况

目的：观察前脑缺血再灌注后海马，纹状体，皮层神经元损伤情况。方法：夹闭Wistar大鼠双侧颈总动脉5min制造脑缺血再灌注模型，应用电镜，Tunel染色观察前脑神经元损伤情况。结果：短暂性脑缺血再灌注第2d，纹状体和皮层神经元坏死，凋亡情况较明显，第4d，海马神经元坏死轻微，凋亡情况最明显，神经元凋亡形态学典型表现持续时间较短，呈色深浅不一。第2d后坏死神经元数量不再增加。结论：脑缺血可致神经元坏死和凋亡，凋亡持续时间较短，以后细胞的形态改变与坏死近似，不同神经元耐受缺氧能力差异很大。

Neuronal reperfusion damage after forebrain ischemia in rats at different intervals

Aim: To observe the damage of hippocampus, striatum, and cortical neurons after transient forebrain ischemia in the rats.Methods: Cerebral ischemic models were made by clamping bilateral carotid arteries for 5 min. Neuronal damage was studied by electric microscope and sequential Tunel technique. Results: On the 2nd day after the transient forebrain ischemia, neuronal necrosis and apoptosis in striatum and cotrex were serious. On the 4th day, neuronal necrosis in hippocampus was slight, but neuronal apoptosis was serious. The dyeing of Tunel indicated that the typical apoptosis remained for a short time. The colors of neuronal apoptosis were different. The number of necrotic neurons did not increase from the 2nd day after ischemia. Conclusions: Cerebral ischemia can cause neuronal necrosis and apoptosis. The lasting time for apoptosis is short. After that, the morphological change of neurons is similar to necrosis, and the ability of anti ischemia in different cerebral parts is greatly different.