| 辽宁省炭疽芽胞杆菌MLVA分型研究 |
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| 引用本文： | 毛玲玲,田疆,雷露,刘学升,张巍,张眉眉,韩悦,姚文清. 辽宁省炭疽芽胞杆菌MLVA分型研究[J]. 中国人兽共患病杂志, 2015, 31(3): 232-234. DOI: 10.3969/cjz.j.issn.1002-2694.2015.03.009 |
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| 作者姓名： | 毛玲玲 田疆 雷露 刘学升 张巍 张眉眉 韩悦 姚文清 |
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| 作者单位： | 1.辽宁省疾病预防控制中心, 沈阳 110005; 2.鞍山市疾病预防控制中心,鞍山 114009; |
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| 基金项目： | 国家科技重大专项艾滋病和病毒性肝炎等重大传染病防治项目(2012ZX10004-209)~~ |
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| 摘 要： | 目的 了解辽宁地区炭疽芽胞杆菌的流行特征及菌型基因特征。方法 通过多位点可变数目串联重复序列(Multiple locus variable numbers of tandem repeats analysis,MLVA)分型实验,对2001—2011年辽宁省分离到的6株炭疽芽胞杆菌分离株DNA进行检测,DNA指纹图谱使用BioNumerics 4.0 软件进行统计分析,得出聚类分析结果。结果 聚类分析发现,6株炭疽芽胞杆菌株可分为2个基因型。对于炭疽暴发而言,其可变数目串联重复序列遗传标记具有高度相似性。结论 炭疽芽胞杆菌基因组中的串联重复序列可作为炭疽芽胞杆菌基因分型的指标,在炭疽暴发事件中的病原体溯源上具有重要的意义。
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| 关 键 词： | 炭疽芽胞杆菌 多位点可变数量串联重复序列分析 基因分型 |
| 收稿时间： | 2014-05-29 |
Bacillus anthracis isolates analysis by multiple-locus variable-numbers of tandem repeats analysis,Liaoning, China |
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| MAO Ling-ling;TIAN Jiang;LEI Lu;LIU Xue-sheng;ZHANG Wei;ZHANG Mei-mei;HAN Yue;YAO Wen-qing. Bacillus anthracis isolates analysis by multiple-locus variable-numbers of tandem repeats analysis,Liaoning, China[J]. Chinese Journal of Zoonoses, 2015, 31(3): 232-234. DOI: 10.3969/cjz.j.issn.1002-2694.2015.03.009 |
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| Authors: | MAO Ling-ling TIAN Jiang LEI Lu LIU Xue-sheng ZHANG Wei ZHANG Mei-mei HAN Yue YAO Wen-qing |
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| Affiliation: | 1. Liaoning Center for Disease Control and Prevention, Shenyang 110005, China; 2. Anshan Center for Disease Control and Prevention, Anshan 114009, China |
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| Abstract: | The epidemic characteristics and genotype of Bacillus anthracis strains in Liaoning Province, China was analyze in this study. Six Bacillus anthracis strains from 2001 to 2011 were studied with multiple-locus variable-number tandem repeat analysis (MLVA). BioNumerics4.0 software was used to analyze the DNA fingerprint of statistics, and cluster analysis results were obtained. Clustering analysis found that the 6 strains could be divided into two genotypes. For anthrax outbreaks, the genetic markers of multiple-locus variable-number tandem repeat were highly similar. It's suggested that MLVA is quite useful for investigation of strain relatedness in regions of outbreaks. |
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| Keywords: | Bacillus anthracis multiple locus variable numbers of tandem repeats analysis genotype |
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