芳香化酶mRNA在肺癌组织中表达的初步研究

目的 探讨芳香化酶Ｐ４５０ ｍＲＮＡ在肺癌组织中的表达及其定位分布情况。方法 以芳香化酶ｃＲＮＡ作探针,采用原位杂交检测２３例肺癌及其相对应的癌旁组织和７例肺良性疾病及其相应正常肺组织中芳香化酶ｍＲＮＡ的表达。结果 １１例肺鳞癌、６例腺癌和２例腺鳞癌芳香化酶ｍＲＮＡ表达均为阳性,而３例小细胞肺癌和１例肺泡细胞癌均为弱阳性。阳性信号主要位于癌细胞的胞浆和胸核内,肿瘤的间质细胞和血管内皮细胞也有少量表

Preliminary study on the expression of aromatase P450 mRNA in human lung cancer tissues

Objective To evaluate the expression, location and distribution of aromatase P450 mRNA in human lung cancer. Methods Using aromatase P450 cRNA as probe, the expression of aromatase mRNA were tested in 23 patients with primary lung cancer and 7 patients with pulmonary benign diseases by in situ hybridization. Results Positive expression of aromatase mRNA was found in 11 cases with squamous cell carcinoma, 6 cases with adenocarcinoma and 2 cases with adenosquamous carcinoma. 3 cases with small cell lung cancer and 1 case with bronchioloalveolar carcinoma were all weak positive. Positive signals were mainly found in cytoplasm and nuclei of tumor cells, and a small quantity of positive expression of aromatase mRNA was found in stromal cells and vascular endothelium cells of the tumor. But no expression of aromatase mRNA was found in the noncancerous tissues and tissues of benign pulmonary lesions. Conclusion The results suggest that the expression of aromatase mRNA are general in lung cancer tissues, and it might play an important role in the oncogenesis and development of lung cancer.