| 用SDS-PAGE和双向肽谱法比较SRS病毒蛋白和Moloney小鼠白血病病毒蛋白 |
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| 引用本文： | 钟德刚,郑葆芬. 用SDS-PAGE和双向肽谱法比较SRS病毒蛋白和Moloney小鼠白血病病毒蛋白[J]. 复旦学报(医学版), 1987, 0(2) |
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| 作者姓名： | 钟德刚 郑葆芬 |
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| 作者单位： | 上海医科大学基础医学部生物物理学教研室(钟德刚)，上海医科大学基础医学部生物物理学教研室(郑葆芬) |
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| 基金项目： | 中国科学院科学基金,编号:[84]科基生准字第126号 |
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| 摘 要： | 用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法,测定SRS病毒(SRSV)蛋白的分子量,比较SRSV蛋白和Moloney小鼠白血病病毒(M-MuLV)蛋白的电泳速度,发现前者p30比后者p30的电泳速度快,推测SRSV是N-向性病毒。用微晶纤维素薄膜制备两株病毒p30的双向肽谱,显示出一些不同的肽段。结果表明SRS V 和M-MuLV是两株不同的小鼠白血病病毒。
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| 关 键 词： | 白血病病毒 病毒蛋白 聚丙烯酰胺凝胶电泳 肽谱 |
A COMPARISON OF SRS VIRUS PROTEINS & MOLONEY MURINE LEUKEMIA VIRUS PROTEINS BY POLYACRYLAMIDE GEL ELECTROPHORESIS AND PEPTIDE MAPPING |
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| Zhong Degang,Zheng Baofen. A COMPARISON OF SRS VIRUS PROTEINS & MOLONEY MURINE LEUKEMIA VIRUS PROTEINS BY POLYACRYLAMIDE GEL ELECTROPHORESIS AND PEPTIDE MAPPING[J]. Fudan University Journal of Medical Sciences, 1987, 0(2) |
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| Authors: | Zhong Degang Zheng Baofen |
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| Abstract: | The molecular weights of SRS virus (SRSV) proteins were estimated and the SRSV was compared with the Moloney murine leukemia virus (M-MuLV) by sodium dodecyl sulfated-polyacrylamide gel electrophoresis (SDS-PAGE). The SRSV p30 moved slightly faster on the SDS-PAGE than M-MuLV p30, so we suggest that SRSV may be a N-tropic virus. Comparison of SRSV p30 with M-MuLV p30 by two-dimensional tryptio peptide mappings on cellulose-coated thin-layer plates showed that there were some other different peptides between the two p 30 proteins. We conclude that SRSV and M-MuLV are different murine leukemia viruses. |
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| Keywords: | leukemia virus viral protein polyacrylamide gel electrophoresis peptlde mapping |
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