C57BL/6N系小鼠腭裂模型的建立及扫描电镜观察

观察腭裂形成过程及腭突正常发育中形态发生、组织学变化。方法１６只妊娠１０天的Ｃ５７ＢＬ／６Ｎ系小鼠随机分为实验组、对照组、于ＧＤ１３＾１４，（１３ｄ１４ｈ略写为３＾１４以下类同），ＧＤ１３＾３２，ＧＤ１４＾８，ＧＤ１４＾２２，ＧＤ１５＾８，ＧＤ１５＾２２，ＧＤ１６＾８剖腹取鼠头部标本分别做扫描电镜及ＨＥ染色。

The model established of cleft palate in C57BL/6N mouse strians and the observation by SEM

Objective To observe the change of the morphogenetics and histolagical Changes during development of cleft palate and normal palate.Methods 16 pregnant mice were devided into experimental group and control group randomly. The experimental group was dosed retinoic acid (RA) dissolved in vegetable oil.The control group was only dosed vegetable oil. The experinental mice were killed at GD13 14 ,GD13 22 ,GD14 8,GD14 14 ,GD14 22 ,GD15 8,GD15 22 ,GD16 8 respectively.The head of embryonic mice were prepared for Scanning Electron Microscope (SEM) and HE stain.Results In treated group mice,the lift of palate shelves was delayed and small palate shelves developed.Two side palates failed to fuse with each other.Conclusion RA affects the proliferation of the embryonic palate mesenchyme cells.This results in insufficient development of palatal shelves and leads to cleft palate.