| 高效液相色谱法测定芪参补血口服液中维生素E含量 |
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| 引用本文： | 梁懿. 高效液相色谱法测定芪参补血口服液中维生素E含量[J]. 中国药业, 2008, 17(20): 31-32 |
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| 作者姓名： | 梁懿 |
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| 作者单位： | 广西南宁百会药业集团有限公司药物研究所,广西,南宁,530003 |
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| 摘 要： | 目的建立芪参补血口服液的维生素E含量测定方法。方法采用高效液相色谱（HPLC）法，色谱柱为YWG C18柱（250mm×4．6mm，10μm），流动相为甲醇-水（98：2），检测波长284nm，柱温30℃，进样量20μL。结果维生素E质量浓度在31．9～318．8μg／mL范围内与峰面积线性关系良好（r=0．9999，n=5）。维生素E的平均回收率为100．3％，RSD=0．22％（n=6）。结论HPLC法简单、准确，可用于芪参补血口服液的维生素E含量测定。
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| 关 键 词： | 芪参补血口服液 维生素E 含量测定 高效液相色谱法 |
Determination of Vitamin E in QishenBuxue Oral Liquid by HPLC |
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| Liang Yi. Determination of Vitamin E in QishenBuxue Oral Liquid by HPLC[J]. China Pharmaceuticals, 2008, 17(20): 31-32 |
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| Authors: | Liang Yi |
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| Affiliation: | Liang Yi ( Institute of Pharmacology, Nanning Best ＆ Wide Pharmaceutical Co., Ltd., Nanning, Guangxi, China 530003) |
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| Abstract: | Objective To establish the determination method of vitamin E in Qishen Buxue Oral liquid. Methods The content of vitamin E in Qishen Buxue Oral Liquid was determined by HPLC. YWG C18 column （250 mm ×4. 6 mm, 10 μm） was used. The mobile phase was methanol-water （98：2）. The detection wavelength was at 284 nm, the column temperature was 30 ℃, the injection volume was 20 μL. Results The calibration curve was linear in the range of 31.9- 318.8 μg/mL for vitamin E （r =0. 999 9, n = 5）. The mean recovery rate of vitamin E was 100.3%, RSD=0. 22% （n=6）. Conclusion This method is simple, reliable and accurate. It can be used to determinate the content of vitamin E in Qishen Buxue Oral Liquid. |
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| Keywords: | Qishen Buxue Oral Liquid vitamin E content determination HPLC |
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