蜂胶黄酮对卡介苗联合脂多糖诱导小鼠免疫性肝损伤的保护作用

目的 探讨蜂胶黄酮(PF)对卡介苗(BCG)与脂多糖(LPS)诱导小鼠免疫性肝损伤的作用.方法 除正常对照组外,采用BCG和LPS诱导小鼠建立免疫性肝损伤模型,随机分为5组,药物实验组分别用300 ms/ks、150 mg/ks、75 mg/ks的蜂胶黄酮干预后,计算肝指数;检测血清中谷丙转氨酶(ALT)和谷草转氨酶(...

The protective effect of propolis flavonoids on immunological liver injury in mice

Objective To investigate the protective effect of the propolis flavonoids on the BCG and LPS-induced immunological liver injury in mice. Methods Kunming mice were divided into control group,model group, bifendate group , low-dosage, middling-dosage, and high-dosage propolis flavonoids groups. On the first day, the model group , bifendate group and the protection groups were injected BCG from tail vein and then the protection groups were orally administered with propolis flavonoids (300 mg/kg,150 mg/kg,75 mg/kg)daily, and the bifendate group were orally administered with bifendate (150 mg/kg) daily. At the end of 12day, all mice were injected with LPS via the tail vein except control groups. The mice were weighed 16h later.The serum alanine aminotransferase (ALT) and aspartate transaminase (AST) activities were determined and the mice were scarified to calculate liver somatic index. Liver homogenate were prepared and liver tissue ma-londialdehyde (MDA) content and erythrocuprein (SOD) and glutathione peroxidase (GSH-Px) activities were tested. Results Propolis flavonoids groups reduced the somatic index(F=9. 88, P<0. 05) ,and decreased serum ALT and AST activities of liver of mice compared with the model group (F=55. 66 or 6. 86, P <0.05) and also decreased MDA content (F=8. 61, P<0. 05 ), increased SOD and GSH-Px activities, compared with the model group (F=11. 96 or 11. 14, P<0.05) . Conclusion Propolis flavonoids protected the BCG and LPS-induced immunological liver injury in mice.