不同程度间歇低氧对大鼠血清炎性细胞因子白介素8、白介素10的影响

目的 探讨不同程度间歇低氧对大鼠血清炎性细胞因子白介素8(IL-8)和抗炎细胞因子IL-10水平的影响.方法 160只雄性Wistar大鼠随机分为5组:5%,7.5%,10%间歇低氧组,10%持续低氧对照组和常氧对照组各32只,分别于低氧暴露第2周、第4周、第6周和第8周每组随机抽取8只大鼠,应用酶联免疫吸附试验法检...

Effects of different degree of intermittent hypoxia on serum levels of inflammatory cytokine interleukin-8 and interleukin-10 in rats

Objective To investigate the effects of different degree of intermittent hypoxia on serum levels of pre-inflammatory cytokines interleukin-8 (IL-8) and anti-inflammatory cytokines IL-10 in rats.Methods One hundred and sixty male Wistar rats were divided randomly into the five groups: 5%,7. 5%, 10% of intermittent hypoxia group, 10% sustained hypoxia control group and the normal oxygen control group. At the second, fourth, sixth, and eighth weeks, 8 rats under different environmental stimulation hypoxia in each group were sacrificed to collect serum. Enzyme Linked Immuno Sorbent Assay (ELISA) assay were used to detect the concentration of serum IL-8 and IL-10. Results Serum level of IL-8 was found to be significantly increased in intermittent hypoxia and sustained hypoxia groups ( F =6. 42, P ＜0. 01) with progression of time, while level of IL-10 was significantly decreased ( F =5. 787,P＜0. 01). We identified the peak for serum levels of IL-8 and IL-10 in the sixth week in all three intermittent hypoxia groups. Serum levels of IL-8 were higher in 5% , 7. 5% ,10% of intermittent hypoxia group than in 10% sustained hypoxia control group and the normal oxygen control group groups ( P ＜0. 01), whereas levels of IL-10 were significantly lower than 10% sustained hypoxia control group and the normal oxygen control group groups ( P ＜0. 01). Level of IL-8 in 5% intermittent hypoxia group was significantly higher than those in 10% intermittent hypoxia group group ( F =34.68, P = 0. 046).Conclusions Intermittent hypoxia and sustained hypoxia can result in elevation of pro-inflammatory cytokines IL-8 and descent of anti-inflammatory cytokine IL-10. Inflammatory response induced by intermittent hypoxia had more serious implications than that by 10% sustained hypoxia control group.Furthermore the release of inflammatory factors is associated with the degree and time of intermittent hypoxia.