垂体中叶素对去甲肾上腺素诱导乳鼠心肌细胞凋亡的影响

目的 观察垂体中叶素(IMD)对去甲肾上腺素(NE)诱导的乳鼠心肌细胞凋亡的影响.方法 20孔原代培养的乳鼠心肌细胞完全随机分为5组,各4孔:正常对照组、NE组、IMD组、降钙素基因相关肽(CGRP)8-37组和肾上腺髓质素(ADM)22-52组.NE组加入1×10-6mol/L NE,IMD组在加NE前30 min加入1×10-8mol/L IMD,CGRP8-37组和ADM22-52组在加IMD前30 min分别加入1×10-7mol/L CGRP8-37与1×10-7mol/L ADM22-52.采用流式细胞仪检测心肌细胞凋亡率并测定半胱氨酸天冬氨酸蛋白酶(Caspase)-3活性.结果 与正常对照组比较,NE组心肌细胞凋亡率和Caspase-3活性均升高[分别为(31.95±6.33)%比(8.70±3.92)%,(2.97±0.20)%比(1.00±0.15)%,均P＜0.05];IMD组的凋亡率和Caspase-3活性[分别为(11.57±4.69)%、(1.34±0.18)%]比NE组明显降低(P＜0.05);CGRP8-37组和ADM22-52组细胞凋亡率[分别为(21.84±5.48)%、(23.43±6.54)%]和Caspase-3活性[分别为(2.15±0.23)%、(2.31±0.26)%]比IMD组明显增高(P＜0.05).结论 IMD通过与CGRP受体和ADM受体结合抑制乳鼠心肌细胞凋亡,对NE诱导的心肌细胞损伤具有防护作用.

Abstract：

Objective To investigate the effect of intermedin (IMD)on neonatal rat cardiomyocytes apoptosis induced by noradrenaline (NE). Methods Totally 20 cultured neonatal rats cardiomyocytes were randomly divided into S groups:control group, NE group, IMD group,calcitonin-gene-relatedpeptide(CGRP)8-37 group and adrenal medulla(ADM)22-52 group. Cultured cardiomyocytes were given 10-6 mol/L NE. At 30 min before administration of NE, IMD was added into the culture fluid in IMD group. CGRP8-37 or ADM22-52 were added at 30 min before IMD administration in CGRP8-37 group and ADM22-52 group respectively. Cardiomyocvte apoptotic rate (AR) and Caspase-3 activity were detected. Results Compared with control group, AR and Caspase-3 activity increased in NE group[(31.95±6.33)% vs (8.70±3.92)%,(2.97±0.20)% vs (1.00±0.15)% ,P＜0.05]. AR and Caspase-3 activity decreased significantly in IMD group compared with NE group(P＜0.05). AR and Caspase-3 activity were higher in CGRP8-37 group and ADM22-52 group than those in IMD group (P＜ 0. 05). Conclusion IMD can attenuate cardiomyocyte apoptosis induced by NE through CGRP receptor and ADM receptor.

Effect of intermedin on noradrenaline induced neonatal rat cardiomyocytes apoptosis

Objective To investigate the effect of intermedin (IMD)on neonatal rat cardiomyocytes apoptosis induced by noradrenaline (NE). Methods Totally 20 cultured neonatal rats cardiomyocytes were randomly divided into S groups:control group, NE group, IMD group,calcitonin-gene-relatedpeptide(CGRP)8-37 group and adrenal medulla(ADM)22-52 group. Cultured cardiomyocytes were given 10-6 mol/L NE. At 30 min before administration of NE, IMD was added into the culture fluid in IMD group. CGRP8-37 or ADM22-52 were added at 30 min before IMD administration in CGRP8-37 group and ADM22-52 group respectively. Cardiomyocvte apoptotic rate (AR) and Caspase-3 activity were detected. Results Compared with control group, AR and Caspase-3 activity increased in NE group[(31.95±6.33)% vs (8.70±3.92)%,(2.97±0.20)% vs (1.00±0.15)% ,P＜0.05]. AR and Caspase-3 activity decreased significantly in IMD group compared with NE group(P＜0.05). AR and Caspase-3 activity were higher in CGRP8-37 group and ADM22-52 group than those in IMD group (P＜ 0. 05). Conclusion IMD can attenuate cardiomyocyte apoptosis induced by NE through CGRP receptor and ADM receptor.