不同剂量白酒对大鼠心肌影响的研究

目的 观察不同剂量白酒对大鼠心肌损伤标志物及心肌组织的影响并探讨其机制,为临床防治乙醇性心肌病提供理论基础和实验依据.方法 140只2～3月龄健康SD大鼠按随机排列表随机分为对照组及不同剂量白酒灌胃模型组A组0.8 ml/(kg·d)、B组1.6 ml/(kg·d)、C组2.4 ml/(kg·d)、D组3.2 ml/(kg·d)、E组4.0 ml/(kg·d)、F组4.8 ml/(kg·d)],每组20只.对照组每日给予10 ml/(kg·d)生理盐水灌胃,其余模型组分别给予相应浓度白酒灌胃,每周称重1次,灌胃6次,连续灌胃16周.16周后每只大鼠禁食12 h取颈总静脉血检测肌酸激酶同工酶(CK-MB)、肌钙蛋白T(cTnT);处死大鼠取心肌组织进行病理学检查,同时取大鼠心肌组织用TUNEL法检测细胞凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶(Caspase)-3的表达水平.结果 A、B组与对照组相比,CK-MB、cTnT水平差异均无统计学意义(均P＞0.05).C、D、E、F组与对照组及A、B组相比,CK-MB、cTnT水平明显升高,差异均有统计学意义(均P＜0.01).对照组及A～F组大鼠的Caspase-3水平(吸光度值)分别为(93.76±14.76)、(92.49±15.39)、(93.19±13.36)、(94.99±14.33)、(196.67±25.16)、(239.86 ±20.99)、(246.66±23.16).D、E、F组分别与对照组、A、B、C组相比,差异均有统计学意义(均P＜0.05).A、B、C组与对照组相比,Caspase-3水平均差异无统计学意义(P＞0.05).结论 适量0.8～1.6 ml/(kg·d)]饮酒不会引起心肌损伤标志物升高及细胞病理学改变,但过量乙醇摄入可导致心肌酶谱变化、Caspase-3表达增加、心肌细胞凋亡和坏死.

Abstract：

Objective To observe the influence of different doses of alcohol on rat cardiac tissue, to establish a chronic animal model and to explore the prevention and treatment of alcoholic heart muscle disease. Methods Totally 140 SD rats were randomly divided into 7 groups: the normal control group and the different doses lavage alcohol model group. Four months later, Jugular Venous blood was immediately taken from each rat to detect creatine kinase isozymes (CK-MB) , cardiac troponin T (cTnT). The myocardial tissue in vivo from rat was taken for pathological examination to observe the impact of alcohol on them. The expression of apoptosis-related protein product Caspase-3 in the myocardial cells was detected. Results The CK-MB, cTnT of A, B groups had no significant change, but CK-MB, cTnT levels in C, D, E, F groups were significantly increased (P＜0.01). Myocardial edema, myocardial cell degeneration and necrosis in model D, E, F groups had obvious changes, but the myocardial pathological in model A, B, C groups and the control group had significant changes. Caspase-3 expression rates of model of A, B, C groups had no significant differences, but Caspase-3 expression rate in model of D, E, F groups was obviously higher than that in A, B, C groups and control group (P ＜ 0.05). Conclusions Low dose of alcohol does not cause the increase of cardiac markers and the change of cytopathology. However, excessive alcohol consumption can lead to the increase of myocardial enzymes and Caspase-3 expression, furthermore, the myocardial cell apoptosis or necrosis.

The experimental study on the effect of alcohol on cardiac tissue in rats

Objective To observe the influence of different doses of alcohol on rat cardiac tissue, to establish a chronic animal model and to explore the prevention and treatment of alcoholic heart muscle disease. Methods Totally 140 SD rats were randomly divided into 7 groups: the normal control group and the different doses lavage alcohol model group. Four months later, Jugular Venous blood was immediately taken from each rat to detect creatine kinase isozymes (CK-MB) , cardiac troponin T (cTnT). The myocardial tissue in vivo from rat was taken for pathological examination to observe the impact of alcohol on them. The expression of apoptosis-related protein product Caspase-3 in the myocardial cells was detected. Results The CK-MB, cTnT of A, B groups had no significant change, but CK-MB, cTnT levels in C, D, E, F groups were significantly increased (P＜0.01). Myocardial edema, myocardial cell degeneration and necrosis in model D, E, F groups had obvious changes, but the myocardial pathological in model A, B, C groups and the control group had significant changes. Caspase-3 expression rates of model of A, B, C groups had no significant differences, but Caspase-3 expression rate in model of D, E, F groups was obviously higher than that in A, B, C groups and control group (P ＜ 0.05). Conclusions Low dose of alcohol does not cause the increase of cardiac markers and the change of cytopathology. However, excessive alcohol consumption can lead to the increase of myocardial enzymes and Caspase-3 expression, furthermore, the myocardial cell apoptosis or necrosis.