| 鲍曼不动杆菌耐药性监测与外排泵adeB基因表达水平检测 |
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| 引用本文： | 宋晓萍,刘萍萍,孙明娥,孙滨. 鲍曼不动杆菌耐药性监测与外排泵adeB基因表达水平检测[J]. 国际检验医学杂志, 2011, 32(12): 1297-1298. DOI: 10.3969/j.issn.1673-4130.2011.12.012 |
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| 作者姓名： | 宋晓萍 刘萍萍 孙明娥 孙滨 |
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| 作者单位： | 1. 山东省青岛市海慈医疗集团检验科,266033
2. 山东省青岛市第六人民医院检验科,266033 |
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| 摘 要： | 目的 探讨鲍曼不动杆菌临床分离株的耐药性以及外排泵adeB基因的表达情况.方法 琼脂稀释法检测最低抑菌浓度(MIC)、聚合酶链反应(PCR)扩增adeB基因,逆转录PCR(rt-PCR)检测adeB基因的mRNA表达水平.结果 药敏试验结果显示,60株鲍曼不动杆菌对哌拉西林耐药率最高(92.3%),对亚胺培南耐药率最低...
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| 关 键 词： | 鲍氏不动杆菌 抗药性.多药 主动外排系统 adeB基因 |
Investigation of Acinetobacter baumannii drug resistance and the expression of efflux pump adeB gene |
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| Song Xiaoping,Liu Pingping,Sun Ming'e,Sun Bin. Investigation of Acinetobacter baumannii drug resistance and the expression of efflux pump adeB gene[J]. International Journal of Laboratory Medicine, 2011, 32(12): 1297-1298. DOI: 10.3969/j.issn.1673-4130.2011.12.012 |
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| Authors: | Song Xiaoping Liu Pingping Sun Ming'e Sun Bin |
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| Affiliation: | 1(1.Department of Laboratory Medicine,Qingdao Hiser Medical Group,Qingdao Shandong 266033,China;2.Department of Laboratory Medicine,Qingdao Sixth People′s Hospital,Qingdao Shandong 266033,China) |
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| Abstract: | Objective To investigate the drug resistance of Acinetobacter baumannii clinical isolates and the expression of efflux pump adeB gene.Methods Agar dilution method was used to determine the minimal inhibitory concentrations(MIC) of Acinetobacter baumannii,polymerase chain reaction(PCR)was used to amplify the adeB gene and reveres transcription(rt-PCR)was used to detect the mRNA level of adeB gene.Results Of the 60 clinical isolates,the resistant rate to piperacillin was the highest(92.3%) and to impipenem wa... |
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| Keywords: | acinetobacter baumannii drug resistance mutliple active efflux systems AdeB gene |
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