盐酸异丙肾上腺素在急性肺损伤早期的干预作用

目的 探讨肺表面活性物质(PS)系统在油酸性急性肺损伤(ALI)时变化的作用机制及β肾上腺素能受体激动剂的干预作用,为临床治疗ALI开辟新的治疗措施提供理论依据和实践方向.方法 30只SD大鼠随机分成3组(每组10只),即油酸致ALI组(OA组)、异丙肾上腺素治疗组(ISO组)和生理盐水对照组(NS组).观察4 h后测...

Early intervention of isoprenaline hydrochloride in acute lung injury

Objective To investigate the changes of pulmonary surfactant (PS) in acute lung injury (ALI) induced by oleic acid and the intervention effect of β-adrenergic receptor agonists, and to provide a theoretical basis and practical direction for new therapeutic measures in the treatment of ALI. Methods Thirty Sprague-Dawley rats were randomly divided into ALI group induced by oleic acid (OA group, n = 10) , isoprenaline treatment group (ISO group, n =10) , and normal saline control group (NS group, n = 10). After four hours, PaO2 , PaCO2, pH, and the serum surfactant protein A (SP-A) levels were assayed. After right lung was lavaged, total proteins ( TP ), total phospholipids ( TPL), phosphatidylcholine (PC) and SP-A in the bronchoalveolar lavage fluid (BALF) were determined. The left lung was used for measurement of wet/dry weight ratio (W/D), beta-adrenergic receptor (β-AR) ,and histopathology. Results Compared with NS group, TPL, PC/TPL and SP-A in the BALF significantly decreased (all P ＜0. 01), W/D, TP and serum SP-A were increased ( P ＜0. 05 or P ＜0.01) in OA group. The values of SP-A in the BALF were negatively correlated to those in the serum in OA group ( r = - 0. 910, P ＜0. 05). Compared with OA group, TPL, PC/TPL and SP-A in the BALF increased, W/D, TP and serum SPA decreased in ISO group ( P ＜0. 05 or P ＜0. 01). Conclusions The PS inactivation may be an important internal cause during the course of ALI induced by oleic acid. The serum SP-A concentration is significantly elevated in ALI rats, so serum SP-A may be a marker for the early diagnosis of ALI, and β-adrenergic receptor agonists may play some protective role in the early stage of ALI.