脉冲电磁场对破骨细胞和成骨细胞前体细胞作用的研究

目的 研究脉冲电磁场（PEMFs）对破骨细胞和成骨细胞前体细胞的作用。方法单独离体PEMFs作用：取8周龄雌性SD大鼠股骨骨髓细胞，根据PEMFs作用方式分为4个剂量组和对照组．分别进行成纤维细胞集落形成单位（CFU—F）和粒／巨噬细胞集落形成单位（CFU—GM）培养观察。活体结合离体PEMFs作用：取8周龄雌性SD大鼠随机分为3组：2—70组、OVX组和SHAM组，其中2．70组．OVX组进行双侧卵巢切除手术，SHAM组不切除卵巢。术后12周对2—70组大鼠进行PEMFs作用，OVX组和SHAM组不进行PEMFs作用。作用结束后，取大鼠股骨骨髓。根据体外培养过程中是否继续接受PEMFs作用，分为2—70PEMFs作用组／未作用组、OVXPEMFs作用组侏作用组、SHAMPEMFs作用组／未作用组，分别进行CFU．F和CFU．GM培养观察。结果单独离体PEMFs作用时，与对照组相比，4个剂量组的CFU-GM减少，CFU．F增加；活体结合离体PEMFs作用时，剂量组的CFU—F均增加，而CFU—GM各组间均无显著差异。结论单独离体PEMFs作用时，PEMFs对体外培养的破骨细胞前体细胞有抑制作用，对成骨细胞前体细胞增殖有促进作用；活体结合离体PEMFs作用时，PEMFs对成骨细胞前体细胞增殖有促进作用，但未见对破骨细胞前体细胞的抑制作用。

Effects of pulsed electromagnetic fields on the precursor cells of osteoclasts and osteoblasts

Objective To study the in vitro and in vivo effects of pulsed electromagnetic fields (PEMFs) on osteoclast and osteoblast precursor cells.Methods To observe the in vitro effect of PEMFs,femur bone marrow cells of 8 week old female SD rats were collected.According to different treating doses,rats were divided into four treatment groups and one control group.After the treatment,the clones of granuloeyte/maerophage colony forming unit(CFU-GM) and fibroblast colony forming units(CFU-F) were measured respectively.To observe the combined in vitro and in vivo effect of PEMFs,8 week old female SD rats were randomly divided into three groups: 2-70 group,ovariectomization (OVX) group and SHAM group.Rats in the 2-70 group and OVX group were bilateral ovariectomized,while rats in the SHAM group were sham-ovariectomized.12 weeks after ovariectomization,the 2-70 group was exposed to PEMFs while the other groups were left untreated.Then,femur bone marrow cells of the rats were collected.According to the way whether the groups were treated with PEMFs,the cells were divided into six groups: 2-70 with/without treatment,OVX with/without treatment,SHAM with/without treatment.After the treatments,the clones of CFU-GM and CFU-F were measured respectively.Results in vitro effect of PEMFs: Compared with the control group,the CFU-GMin the treated groups reduced while the CFU-F increased.PEMFs effect in vitro and in vivo: The CFU-F in treated groupsincreased,whileno.significantdifferencesofCFU-GMwerefoundamongthegroups.Conclusion PEMFs has inhibitory effect on osteoelast precursor cells and enhances the proliferation of osteoblast precursor cells when simply applied in vitro.When PEMFs was applied in combined manner of in vitro and in vivo,it shows that PEMFs enhance the proliferation of osteoblast precursor cells but has no inhibitory effects on osteoelast precursor cells.