抑癌蛋白维生素D3上调蛋白1在肺癌组织中的表达及意义

目的 探讨肿瘤抑制蛋白维生素D3上调蛋白1(vitamin D3 up-regulated protein 1,VDUP1)在肺癌组织中的表达及其与肺癌临床、病理之间的关系和意义.方法 应用RT-PCR及Western blot分别检测35例肺癌患者的癌组织及癌旁正常组织VDUP1 mRNA和蛋白表达水平,进一步按照癌...

Expression and significance of tumor suppressor vitamin D3 up-regulated protein 1 in lung cancer

Objective To investigate the expression of tumor suppressor vitamin D3 up-regulated protein 1 (VDUP1) in lung cancer, and to reveal its relationship with lung cancer and significance.Methods The expressions of VDUP1 mRNA and protein in lung cancer tissues and adjacent normal lung tissues of 35 patients with lung cancer were detected by real time-polymerase chain reaction and Western blot. The hierarchical comparison was further performed according to cancer cell differentiation and TNM clinical stage of lung cancer. Results The expressions of VDUP1 mRNA and protein in lung cancer tissues were obviously lower than those in adjacent normal lung tissues ( P =0. 000). The expression of VDUP1 protein in lung cancer was related to TNM stage and cell differentiation (all P <0. 05), but not to smoking, sex, and histological type. Multiple analysis results showed VDUP1 expression was respectively 1. 17±0. 07,0. 91±0. 26,0. 83±0. 26 in high, middle, and low differentiation lung cancer tissues ( P =0. 023). VDUP1 expression at stage Ⅰ~Ⅱ(1. 03±0. 20) was significantly higher than that at stage Ⅲ ~Ⅳ (0. 79±0. 24, P =0. 004). Conclusions VDUP1 is lowly expressed in lung cancer, and related with TNM stage of lung cancer and cell differentiation, may be an effective indicator to determine progress and prognosis of lung cancer.