全反式维甲酸对结肠癌CW-2和LS174T细胞株增殖的抑制作用研究

目的：研究全反式维甲酸(ATRA)对结肠癌不同增殖能力细胞株生长抑制的可能机制，为ATRA应用于结肠癌的治疗提供可靠依据。方法：应用细胞观察和MTT方法，研究ATRA对结肠癌不同增殖能力细胞株LS174T和CW-2细胞的生长抑制现象。结果：LS174T的生长速度明显比CW-2细胞快。ATRA对体外培养的结肠癌细胞株LSl74T和CW-2细胞的生长有明显抑制和诱导细胞分化作用。结论：ATRA对LS174T和CW-2细胞的生长有抑制作用，抑制细胞增殖的程度与ATRA的浓度有关，ATRA对结肠癌细胞有一定的抑制癌细胞增殖和诱导癌细胞分化作用。

THE STUDY OF INHIBITION PROLIFERATION OF CELL STRAIN WITH DIFFERENT PROLIFERATIVE POTENTIAL IN CLOLRECTAL BY ALL TRANS REINOIC ACID

Objectioe:To study the inhibition of mechanism of cell strain proliferation with different proliferative potential in colorectal carcinoma by ATRA in order,to provide the base of therapy colorectal carcinoma by ATRA.Mehtods: Using cell observation and MTT method,to study the phenomena that proliferation of LS174T and CW-2 cell strain were inhibited by ATRA. Results: Proliferation of LS174T cell strain was faster than those of CW-2 .ATRA played a role in inhibition of proliferation and induction of cell differentiation to LS174T and CW-2 cell strain of colorectal carcinoma in vitro. Conclusion: ATRA palys a role in inhibitrion proliferation of LS174T and CW-2 cell strain,and the inhibition ability is correlated with concentration of ATRA, ATRA may inhibit cell proliferation and induce cell differentiation to cell of colorectal carcinoma.