宣威昆明两地非小细胞肺癌患者P16蛋白表达的比较研究

目的研究宣威、昆明两地非小细胞肺癌患者P16蛋白表达的差异性,探讨P16蛋白在肺癌发生发展中的作用。方法采用免疫组化对宣威、昆明地区非小细胞肺癌各45例和非肺癌(肺大疱、肺出血)行手术切除的正常肺组织石蜡包埋标本18例进行P16蛋白表达检测。结果 P16蛋白免疫组化阴性表达共有45例,总失活率为41.7%,其中宣威肺癌组、昆明肺癌组、对照组的失活率分别为57.8%、37.8%、11.1%。两肺癌组P16蛋白失活率分别与对照组的差异有统计学意义(P<0.05);两肺癌组P16蛋白的总失活率差异无显著性(P=0.058);宣威肺癌组在Ⅰ期、T2期和腺癌的失活率比昆明肺癌组高(P<0.05)。结论与昆明肺癌组相比,宣威肺癌组P16蛋白不表达率在肺癌早期和腺癌中呈高表达。

Comparative study of expressions of P16 protein in patients suffered from non-small-cell-lung-cancer of Xuanwei and Kunming district

Objective To study the effect and function of P16 protein during the development of lung cancer,in order to explore the differences of expression of P16 protein in non-small cell lung cancer(NSCLC) between Xuanwei and Kunming district.Methods 45 patients with NSCLC from Xuanwei and 45 patients from Kunming,18 patients from pneumatocele who underwent radical resection were collected.Immunohistochemical staining were used to analyze the expressions of P16 protein in paraffin-embedded tissues from these 90 residing patients with lung cancer.Results 45 cases with lung cancer were negative for P16 protein expression,total loss expression rates were 41.7%(45 /108).Loss expression rates of Xuanwei,Kunming and pneumatocele were 57.8%(26 /45),37.8%(17 /45),11.1%(2 /18),respectively.In comparition the loss expression of P16 protein in clinicopathologic characteristics between the two groups,there were some statistically significant differences in the loss expression rate of P16(P < 0.05):the loss expression rate of P16 in cases with stage Ⅰ,adenocarcinoma and stage T2 in Xuanwei were more higher than those in Kunming;the loss expression rates of P16 in Xuanwei and Kungming were more higher than those in pneumatocele respectively.Conclusion Loss expression rates of P16 protein in the early stage and adenocarcinoma in Xuanwei lung cancer group were significant difference compared with in Kunming lung cancer group.