奥沙利铂联合多烯磷脂酰胆碱对胃癌细胞增殖影响研究

目的：观察奥沙利铂联合多烯磷脂酰胆碱对胃癌细胞增殖的影响。方法：不同浓度奥沙利铂（0．5、1、2、4、8和16μmol／L）和多烯磷脂酰胆碱（2、4、8、16、32、64和128μmol／L）分别作用于胃癌细胞sGc790124、48和72h，MTT法检测各组肿瘤细胞的生存率。根据公式求出奥沙利铂48h的半抑制浓度（IC50），用略低于此浓度的奥沙利铂与不同浓度的多烯磷脂酰胆碱联合作用于胃癌细胞48h，MTT法检测细胞的生存率，计算2种药物联合的指数。结果：不同浓度的多烯磷脂酰胆碱对肿瘤细胞增殖产生了促进或者抑制作用，促进增殖或者抑制增殖作用与药物浓度具有明显相关性，F=373．769，P〈0．001；但与药物作用时间无明显统计相关，F=0．077，P=0．782。奥沙利铂抑制肿瘤增殖，这一作用也表现出明显的剂量=时间相关性，F-194．193，P〈0．001；F=12．428，P=0．001。联合作用组中，不同浓度的多烯磷脂酰胆碱与奥沙利铂联合较单药奥沙利铂相比，均对肿瘤细胞表现出现较高的抑制率，差异有统计学意义，F=297．889，P〈0．001；其中较低浓度多烯磷脂酰胆碱（2～8μmol／L）与奥沙利铂产生了协同抗肿瘤作用（Q〉1．15），高浓度多烯磷脂酰胆碱（32～128／μmool／L）与奥沙利铂发现拮抗反应（Q〈0．85）。结论：奥沙利铂抑制肿瘤细胞增殖，低浓度的多烯磷脂酰胆碱促进肿瘤细胞增殖，高浓度的多烯磷脂酰胆碱抑制肿瘤细胞增殖。奥沙利铂与低浓度多烯磷脂酰胆碱产生了协同抗肿瘤作用，与高浓度多烯磷脂酰胆碱却产生了拮抗抗肿瘤作用。

Effect of oxaliplatin combined with polyenephosphatidylcholine on proliferation of gastric cancer cells

OBJECTIVE：To investigate the effect of combined oxaliplatin（L-OHP） and polyenephosphatidylcholine （PPC） on proliferation of gastric cancer cell line. METHODS：The proliferation rate of different concentrations of PPC and L-OHP on SGC7901 gastric cancer ceils for 24,48,72 hours were examined by MTT and then the half inhibitory concen tration （IC50） of L-OHP acting for 48 hours was calculated. SGC7901 cells were treated with oxaliplatin 3.5 μg/mL and PPC 0 to 128/μmol/L for 48 hours,then proliferation rates were determined by MTT assay and drug combination analysis were calculated according to the formula. RESULTS： MTT test results showed that different concentrations of PPC signifi cantly promoted or inhibited the proliferation of tumor cells,its role in the promoting of proliferation or inhibiting the pro liferation was drug concentration related （F=373. 769,P〈0. 001）,but not drug-action time related（F=0.077,P= 0. 782）. L-OHP inhibited tumor proliferation,its role also demonstrated dose（F= 194. 193, P（0. 001） -time（F= 12. 428, P= 0. 001）-related significantly. Compared with single-agent oxaliplatin, different concentrations of polyenephosphatidyl- choline combined with oxaliplatin all showed higher inhibition rates, the result was significant （F=297. 889, P〈0. 001）; L-OHP and low concentrations of PPC（2--8/μmol/L） showed a synergistic anti-tumor effect （Q〉1 15）while L-OHP and high concentrations of PPC （ 32 - 128μmol/L） produced antagonism （ Q 〈0.85 ）. CONCLUSIONS ： L OHP has a clearantiproliferative effect on SGC7901 gastric cancer cells. Low concentrations of PPC promote tumor cell proliferation,while high concentrations of PPC inhibit tumor proliferation. When two drugs are combined, L-OHP and tow concentrations of PPC show a synergistic anti-tumor effect while L-OHP and high concentrations of PPC have produced antagonism.