大鼠结肠肿瘤组织WnVPCP信号通路相关因子表达意义分析

目的：探讨wnt／PCP信号途径中wnt7a、JNK和RhoA蛋白在结肠肿瘤组织中的表达及在结肠癌形成过程中的作用。方法：用MNU灌肠法诱导大鼠形成结肠肿瘤。免疫组化检测wnt／PCP信号途径中重要因子wnt7a、JNK和RhoA在结肠癌组织、结肠非典型增生组织和正常结肠组织中的表达情况。结果：wnt7a蛋白在结肠非典型增生组阳性表达率为0．713±0．025，高于正常结肠组织组（对照组）的0．208±0．0025，P〈0．001；结肠癌组阳性表达率为0．709±0．015，高于正常结肠组，P〈0．001；而结肠非典型增生组与结肠癌组比较，差异无统计学意义，P=0．892。JNK蛋白在非典型增生组阳性表达率为0．170±0．009，高于正常结肠组的0．682±0．022，P〈0．001；结肠癌组阳性表达率0．674±0．034，高于正常结肠组，P〈0．001；而结肠非典型增生组与结肠癌组比较，差异无统计学意义，P=0．783。RhoA蛋白在结肠非典型组阳性表达率为0．714土0．021，高于正常结肠组的0．270±0．0008，P〈0．001；结肠癌组织中阳性表达率为0．724±0．007，高于正常结肠组，P〈0．001；而结肠非典型增生组与结肠癌组比较，差异无统计学意义，P=0．158。结论：Wnt／PCP信号途径中Wnt7a、JNK和RhoA蛋白在结肠非典型增生和结肠癌组织中高表达，可能与结肠癌的发生发展有密切关系。

Expression of Wnt7a,JNK and RhoA in Wnt/PCP signal pathway in the tissue of rat colonic neoplasms

OBJECTIVE： To explore the expression of Wnt7a,JNK and RhoA in Wnt/PCP signaling pathways in the co lonic neoplasms tissue,and the function of the processing mechanism. METHODS： The colonic neoplasms model of SD rats was established through the MNU enema method. The immunohistochemical method was used to detect the expression of the Wnt7a, JNK and RhoA in colon atypical hyperplasia,colonic neoplasms and normal colon tissues. RESULTS： The positive rate of expression of Wnt7a protein in tissue of the colon atypical hyperplasia group（0. 713!0. 025） was significantly higher than those of the normal colon group（control group） （0. 208±0. 002 5） ,P〈0. 001;The positive rate of expression of WntTa protein in tissue of the colonic neoplasms group（0. 709±0. 015）was significantly higher than that of the control group, P〈0. 001. The positive rate of expression of JNK protein in the tissue of the colon atypical hyperplasia group（0. 170±0. 009） was significantly higher than that of the control group （0. 682 i 0. 022）, P〈0. 001 ;The positive rate of expression of JNK protein in tissue of the colonic neo plasms group （0. 674±0. 034） was significantly higher than that of the control group,P〈0. 001. The positive rate of expression of RhoA protein in tissue of the colon atypical hyperplasia group （0. 714±0. 021） was significantly higher than that of the control group （0. 270±0. 000 8）, P〈0. 001 ; The positive rate of expression of RhoA protein in tissue of the colonic neoplasms group （0. 724±0. 007） was significantly higher than that of the control group,P〈0. 001. The positive rate of expression of the WntTa in tissues of the atypical hyperplasia （0. 713±0. 025） and colonic neoplasms groups （0. 709±0. 015） had no obvious difference, P=0. 892. The positive rate of expression of JNK protein in tissues of the atypical hyperplasia （0. 674±0. 034） and colonic neoplasms groups （0. 682±0. 022） had no obvious difference,P=0. 783. The positive rate of expression of RhoA protein in tissues of the atypical hyperplasia （0. 714±0. 021） and colonic neoplasms groups （0. 724±0. 007） had no obvious difference,P=0. 158. CONCLUSION：The positive rate of expression of WntTa, JNK and RhoA protein in Wnt/PCP signaling pathway in colonicneoplasms and colon atypical hyperplasia tissues is higher than that in the normal colon tissue. These factors probably plays an important role in the forming process of colonic neoplasms.