乳腺癌组织HR与HER-2和SATB1表达相关性研究

目的：检测乳腺癌组织中激素受体（hormonereceptor，HR）、人表皮生长因子受体2（humanepidermalgrowthfactOrreceptor2，HER-2）和特异性核基质结合蛋白（specialATrichsequencebindingprotein1，SATB1）的表达，探讨HR与HER-2、SATB1及临床病理参数的关系。方法：应用免疫组织化学方法检测乳腺癌患者雌激素受体（estrogenre-ceptor，ER）、孕激素受体（progesteronereceptor，PR）、HER-2及SATB1蛋白的表达，荧光原位杂交方法检测HER-2基因扩增状态，统计学方法分析HR（包括ER及PR）的表达与HER-2、SATB1及临床病理参数之间的相关性。结果：乳腺癌组织ER及PR的表达均与患者年龄正相关（r=0．286，P=0．010；r=0．249，P=0．026），ER的表达与肿瘤分级负相关（r=-0．306，P=0．006）；ER及PR的表达与肿瘤大小、组织学类型、淋巴转移情况及TNM分期均无明显相关性，P值均〉0．05。HR的表达与SATB1、HER-2及SATB1／HER2双阳性表达均呈负相关关系（r=-0．248，P=0．027；r=-0．392，P〈0．001；r=-0．150，P〈0．001）。结论：HR阳性患者治疗及预后相对较好；乳腺癌组织HR的表达与HER-2和SATB1呈负相关关系，三者之间可能存在相互联系的信号通路。

Expression of HR and its correlations with HER-2 and SATB1 in breast cancer tissues

OBJECTIVE：To detect the expression of hormone receptor （HR） ,human epidermal growth faclor recep tor 2 （HER 2） and specific nuclear matrix binding protein I（SATB1） in breast cancer,and explore the correlations be tween HR and HEP- 2,SATB1 as well as the elinicopathological features. METHODS： Immunohistoehemistry was used to detect the expression of estrogen receptor （ER）, progesterone receptor （PR）, HER 2 and SATB1 protein in breast cancer patients. FISH was used to test the amplification of HER-2 gene. Correlations of HR with HER-2, SATB1 and clinicopathologic features were analyzed. RESULTS： The expression of ER, PR was positively correlated with patient age in breast cancer（r=0. 286, P= 0. 010;r= 0. 249,P 0. 026）, while ER expression was negatively correlated with tumor grade（r=-0. 306, P = 0. 006）. No correlations were found between ER or PR expression and tumour size, pathological type,lymph node status as well as TNM stage （All P〈0.05）. HR expression was negatively correlated with HER-2, SATB1 expression and SATB1/HER-2 co-expression（r= 0.248,P=0.027;r=-0.392,P〈0.001;r=-0.450,P〈 0. 001）. CONCLUSIONS： HR positive expression may indicate a better therapitic and prognostic outcome. Negative correlations existed between the expression of HR and SATB1/HER 2 in breast cancer tissues; and the phenomenon possibly suggested their cross-talks though the signaling pathways.