骨髓增生异常综合征原癌基因Bmi-1表达的检测

目的：检测原癌基因Bmil在骨髓增生异常综合征（myelodysplastic syndrome，MDS）患者中的表达，探讨其在MDS中的临床意义。方法：选择201009—04—2013—01-10在泰山医学院附属医院就诊的69例MDS患者及30例非恶性血液病患者骨髓标本，应用SYBRGreen相对定量RT—PCR方法，进行Bmi-1基因的检测。结果：Bmi—1基因在非恶性血液病患者中无表达。在69倒MDS患者中均有表达（2．28±0．84），且表达水平明显高于非恶性血液病患者组，t=14．787，P〈0．001。其中，RA组明显高于对照组（P〈0．001），RAEB组明显高于RA组，P=0．001。Bmil基因高表达组CD34＋CD38-CD123＋／CD34＋为（10．77±5．51）％，低表达组为（4．65±3．36）％，差异有统计学意义，t=5．741，P=0．037。Bmi-1基因高表达组，骨髓原始细胞≥5％的病例数（17例）高于低表达组（14例），χ2=7．057，P=0．012；Bmi1基因高表达组具有预后不良染色体核型的有6例，Bmi-1低表达组仅有3例，但两组间差异无统计学意义，χ2=2．794，P=0．144。结论：MDS患者的骨髓单个核细胞中均存在Bmi-1基因不同程度的表达升高，为MDS的诊断及预后的评价提供了新的方向。

Expression of proto-oncogene Bmi-I in myelodysplastic syndrome

OBJECTIVE： To analyze the expression of B-cell-specific moloney murine leukemia virus insertion site 1 （Bmi-1）gene in patients who suffered from myelodysplastic syndrome （MDS）,and explore the clinical significance of the gene. METHODS： The Bmi 1 gene expression levels were detected by SYBR Green reahive-quantitative PCR in bone mar row of 69 MDS patients and 30 cases without malignant hematosis chosed from Sep. 4th, 2010 to Jan. 10th, 2013. RE- SUI,TS： Bmi-1 gene was expressed in all MDS patients （2.28± 0.84） and was not expressed in normal controls. Compared with that in control group,Bmi-1 gene expression was significantly increased in MDS patients（t=14. 787,P〈.0. 001）. The gene level of RA was 2.02±0.51 ,and the level of RAEB was 2.60± 1.05. The difference between them was statistically significant（P= 0.001 ）. The percent of CD34＋CD38-CD123 ＋/CD34＋ in those patients who had a higher level of Bmi 1 gene was （10.77 ± 5.51 ）%, and it showed a notable increase. The cases showing that the proportion of bone marrow orig inal cells ≥5% were also significantly higher in the level of Bmi 1 gene（χ2=7. 057,P=0. 012）. The cases with poor kary otype in higher expression level of Bmi 1 gene were 6, while the cases in lower expression were only 3 and there was no statistical difference（χ2 =2. 794,P=0. 144）. CONCLUSION： Bmi 1 gene expression is increased with different levels in MDS,which provides novel insights into the diagnosis and prognosis of MDS.