阿卡波糖对糖耐量减低大鼠FFA、IGF-1及大血管的影响

目的研究阿卡波糖对糖耐量减低大鼠血清游离脂肪酸（free fatty acid,FFA）、胰岛素样生长因子-1（insulin-like growth factor-1,IGF-1）及大血管病变的影响,探讨阿卡波糖对改善糖耐量减低大鼠大血管病变的作用机理。方法健康雄性SD大鼠40只随机分为正常对照组（n=10）、模型对照组（n=15）、阿卡波糖组（n=15）,后两组在高脂饲料喂养20 d后,予以小剂量链脲佐菌素25 mg/kg腹腔注射制备糖耐量减低大鼠模型。成模后,模型对照组继续高脂饲料喂养,阿卡波糖组给予100 g高脂饲料中加入阿卡波糖40 mg喂养。8周后处死,ELISA法检测血清FFA、IGF-1,同时光学显微镜下观察各组大鼠胸主动脉的病理改变。结果模型对照组FFA较正常对照组、阿卡波糖组升高,均有统计学意义（P〈0.05）,而阿卡波糖组较正常对照组有差异,但无统计学意义（P〉0.05）;模型对照组IGF-1较正常对照组、阿卡波糖组下降,均有统计学意义（P〈0.05）,而阿卡波糖组较正常照组下降,无统计学意义（P〉0.05）。3组大鼠FFA与IGF-1相关系数分别为-0.67、-0.76、-0.60,均呈显著负相关（P〈0．05）。光学显微镜下模型组胸主动脉内皮细胞明显发生改变，阿卡波糖组变化不明显。结论阿卡波糖可以改善糖耐量减低时期大血管病变可能与其调节血清FFA和IGF-1的水平有关。

Effect of Acarbose on FFA, IGF-1 and vascular disease in impaired glucose tolerance rats

Objective To probe the effect of Acarbose on serum free fatty acid （FFA）, insulin-like growth factor-1 （ IGF-1 ） and vascular disease in rat models with impaired glucose tolerance in order to understand the mechanism and improve the treatment of vascular disease. Methods Impaired glucose tolerance rat model was induced by intravenous injection with a low dose of streptozotocin （ 25 mg/kg） and high fat diet, and the model rats were then randomized into two groups： the model control group （n = 15） and the Acarbose group （ n = 15 ）, while 10 normal rats were assigned as the normal control group. Rats in the normal control group were fed with nomal diet, rats in the model control group with high fat diet, and those in the Acarbose group with Acarbose 40 mg/100 g in high-calorie diet. All rats were sacrificed after g weeks, and ELISA was used to measure the rat serum FFA and IGF-1. The pathological changes in thoracic aorta were observed under light microscope. Results Compared with those in the normal control and Acarbose groups, serum FFA in rats of the model control group was significantly higher （ P 〈 0.05 ）, but there was no significant difference between the rats of normal control group and those of the Acarbose group （ P 〉 0.05 ）. Compared with rats of the normal control and the Acarbose groups, IGF-1 in rats of the model control group was significantly lower （ P 〈 0.05 ）, but there was no significant difference between the normal control group and the Acarbose group （ P 〉 0.05 ）. Serum FFA negatively correlated with serum IGF-1 of all the three groups （ r = - 0.67, - 0.76, -0.60, P 〈 0.05 ）. Compared with rats of the normal control group, the morphological changes of the endothelial cell of thoracic aorta in rats of the model control group was significant, but not in those of the Acarbose group. Conclusion It is possible that Acarbose may improve macro vascular disease during the impaired glucose tolerance period by regulating serum FFA and IGF-1 levels.