Letters to the editor |
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Authors: | Walter Merricks Olivia Montuschi Alison Murdoch |
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Institution: | 1. Donor Conception Network, PO Box 265, Sheffield, S3 7YX;2. Bioscience Centre, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP |
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Abstract: | Objective: To describe the birth achieved from frozen embryos after intracytoplasmic sperm injection (ICSI) of donor sperm into vitrified oocytes.Patient: A 25-year-old woman whose husband was azoospermic undergoing IVF therapy.Methods: Oocytes collected after ovarian stimulation were vitrified, thawed, and fertilized by frozen donor sperm.Results: Nineteen oocytes were vitrified and all survived after thawing. Thirteen of the 19 oocytes that underwent ICSI with donors sperm were successfully fertilized. Twelve embryos were cryopreserved again by conventional slow-freezing protocol because of uterine bleeding on the day of transfer. Three thawed embryos were transferred, and a normal male with an infant karyotype of 46,XY was delivered.Conclusion: This case report demonstrates effective oocyte cryopreservation by vitrification. |
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Keywords: | Birth embryo cryopreservation frozen embryo transfer (FET) human intracytoplasmic sperm injection (ICSI) oocyte vitrification |
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