脊髓损伤大鼠胫骨骨痂中降钙素基因相关肽的改变

目的 研究脊髓损伤后大鼠胫骨骨痂中降钙素基因相关肽（ＣＧＲＰ）的变化及骨痂量的改变。方法 将１００只雌性Ｗｉｓｔａｒ大鼠随机分为２组，Ｔ１０－１１脊髓横断＋右胫骨骨折组和单纯右胫骨骨折组各５０只。术后３、７、１４、２１、２８ｄ分批处死，检查血清碱性磷酸酶（ＡＬＰ），摄右胫骨Ｘ线片行骨痂评分，骨痂行苏木精－伊红（ＨＥ）染色和ＣＧＲＰ免疫组织化学染色。结果 脊髓缶伤组３、７ｄ，ＡＬＰ均显著升高（Ｐ〈０

The Change of valcitonin gene-related peptide in tibial fracture callus after spinal cord injury in rats

Objective To study the relationship between the changes of calcitonin gene-related peptide in tibial fracture callus and yield of callus after spinal cord injury in rats. Methods One hundred female Wistar rats were randomly divided into spinal cord transection plus tibial fracture group ( n =50) and tibial fracture group ( n =50). The animals were sacrificed in batches 3,7,14,21 and 28 days after operation. The activities of ALP in serum of rats were measured. X-ray plane films of the fractured tibias were taken to evaluate score of callus. Callus were subjected to H-E staining and CGRP immunohistochemical staining. Results Activities of ALP in serum of spinal cord injury group were significantly higher than those in tibial fracture group at 3 d and 7 d ( P <0.01). The score of callus in spinal cord injury group was higher than in tibial fracture group at 21 d and 28 d. There were a quantity of fibrous callus and cartilaginous callus formation and few CGRP immunoreactive nerve fibers in callus at early stage after spinal cord injury. Strong immunoreactivity of CGRP was found in osteofenitor cells, chondroblasts, which proliferated in thickened endoperiosteal and amidst callu at 3 d and 7 d in spinal cord injury group. There were few CGRP in callus and a little woven bone callus formation at late stage after spinal injury. Conclusions Expression of CGRP in the callus changed greatly, which caused the change of callus yield after spinal cord injury. It was speculated that fracture healing was regulated by neuropeptide.;