卡介苗接种后婴儿细胞免疫状态研究

【目的】 分析新生儿接种卡介苗(bacillus calmette-guerin,BCG)后细胞免疫功能变化。 【方法】 收集150例BCG接种后结核菌素纯蛋白衍生物(purified protein deriva-tive,PPD)阳性婴儿(接种成功组)和50例BCG接种后PPD阴性婴儿(接种失败组)的外周静脉血,应用ELISA方法测定血清细胞因子IFN-γ和IL-10的含量,评价细胞免疫功能。 【结果】 接种成功组和接种失败组血清细胞因子IFN-γ含量分别为(49.78±12.20)和(38.08±7.68)pg/mL,差异具有统计学意义(P=0.001);血清IL-10含量分别为(3.60±2.00)和(5.71±2.71)pg/mL,差异具有统计学意义(P<0.01)。 【结论】 BCG接种成功(PPD阳性)婴儿的细胞免疫功能增强。

Cell-mediated immunity condition in infants vaccinated with BCG

【 Objective 】 To analyze the change in cell-mediated function in infants vaccinated with BCG.【 Methods 】 Peripheral blood specimen from 150infants with positive PPD test(Group successfully Vaccination,SV) and 50ones with negative PPD test(Group failure vaccination,FV) were collected.The cytokine IFN-γand IL-10concentrations were measured by ELISA to evaluate cell-mediated immunity function after BCG vaccination.【 Results 】 Serum IFN-γconcentrations in Groups SV and FV were(49.78±12.20) and(38.08±7.68) pg / mL,respectively(P=0.001).Serum IL-10contents in Group SV and FV were(3.60±2.00) and(5.71±2.71) pg / mL,respectively(P<0.01).【 Conclusion 】 The infants vaccinated successfully(positive PPD test) had an increasing cell-mediated immunity function.