Wistar大鼠永久性局灶性大脑中动脉阻断脑缺血新模型的建立

在Ｗｉｓｔａｒ大鼠用改良的开颅方法两点阻断大脑中动脉，建立一个新的永久性局灶性脑缺血模型（ＰＭＣＡＯ）。通过大鼠脑梗塞后神经功能状况的观察，明胶－墨汁灌注、ＴＴＣ染色计算机图像分析及病理形态学的方法对模型进行研究评价。Ｗｉｓｔａｒ大鼠大脑中动脉阻断后２４　ｈ，神经功能为２级；模型组梗塞面积稳定，占对侧面积５７±５％，其梗塞灶位于脑皮质和纹状体外侧；病理形态学表现为典型的缺血性改变。结果表明，用本方法阻断大脑中动脉，阻断确切，梗塞灶的大小、位置恒定。这一模型为局灶性脑缺血机制的研究以及治疗药物的疗效观察提供了一个可重复、稳定可靠的动物模型。

A New Model of Focal Permanent Cerebral Ischemia by Occluding the Middle Cerebral Artery in Wistar Rats

A new model of irreversible, focal ischemia was developed in wistar rats by occluding the middle cerebral artery (MCA) in double sites transcranially. The model was evaluated by examining neurologic deficits, glutin ink perfusion, TTC staining, computer image analysis and histopathological examination 24 hours following the occlusion, neurologic deficits were in grade 2 and remarkably consistent infarction size (57±5% of the contralateral) was obtained. The infarction area involved was in striatum and cortex, which showing typical ischemic histopathological changs. The result suggust, that the model with more consistent size and location of infarction would be useful for studying the mechanism of cerebral ischemia and experimental assessment of new therapies for cerebral ischemia.