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| 过量碘对甲状腺细胞组织蛋白水解酶的作用研究 | |
| 引用本文： | 刘小立,徐健,李兰英,孙云,郝丽萍,孙秀发.过量碘对甲状腺细胞组织蛋白水解酶的作用研究[J].营养学报,2006,28(5):427-429,433. |
| 作者姓名： | 刘小立 徐健 李兰英 孙云 郝丽萍 孙秀发 |
| 作者单位： | 1. 深圳市慢性病防治院,深圳,518020 2. 华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉,430030;深圳市疾病预防控制中心,深圳,518020 3. 天津医科大学代谢病医院,天津,300070 4. 华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉,430030 |
| 基金项目： | 国家自然科学基金(No.30230330)，深圳市科技基金(No.200404142) |
| 摘 要： | 目的探讨甲状腺蛋白水解酶在过量碘性甲状腺损伤中的作用。方法选取FRTL细胞系为研究对象,正常组培养基内不加碘,高碘组分别含碘(碘化钾)1、5、10、50、100mmol/L,培养6、12、24、48、72h,荧光分光光度法测定蛋白酶B(CB)活性,Lowry法测定蛋白酶D(CD)活性,荧光定量PCR法测定CB和CD的mRNA水平。结果50mmol/L碘作用12h开始出现CB活性的下降,但是需作用24h始出现CD活性的下降。50mmol/L碘作用24h,高碘组CB、CDmRNA水平均较正常组显著下降。结论CB和CD活性的下降是过量碘导致的甲状腺胶质潴留的重要原因之一。 |
| 关 键 词： | 组织蛋白水解酶 过量碘 Fisher大鼠甲状腺滤泡上皮细胞 |
| 文章编号： | 0512-7955（2006）05-0427-04 |
| 收稿时间： | 2006-03-12 |
| 修稿时间： | 2006-03-12 |
STUDY ON THE EFFECT OF IODINE EXCESS ON CATHEPSINS IN RAT THYROID CELLS |
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| LIU Xiao-li,XU Jian,Li Lan-ying,SUN Yun,Hao Li-ping,SUN Xiu-fa.STUDY ON THE EFFECT OF IODINE EXCESS ON CATHEPSINS IN RAT THYROID CELLS[J].Acta Nutrimenta Sinica,2006,28(5):427-429,433. | |
| Authors: | LIU Xiao-li XU Jian Li Lan-ying SUN Yun Hao Li-ping SUN Xiu-fa |
| Institution: | 1.Chronic Disease Hospital, Shenzhen. 518020;2.Department of Nutrition and Hygiene, Huazhong Science and Technology University Tongji Medical College, Wuhan 430030; 3.Center for Disease Control and Prevention, Shenzhen 518020; 4.Institute of Endocrinology, Tianjin Medical University, Tianjin 300070. |
| Abstract: | Objective: To explore the effect of iodine overdose on cathepsin B and D in thyroidcell line (FRTL). Method: The cells were treated with potassium iodide (0, 1, 5, 10, 50 and 100mmol/L). After treatment 6, 12, 24, 48 and 72 hours, the cells were collected. The activities of cathepsin B (CB) and cathepsin D (CD) were assayed by fluorospectrophotometry and Lowry, respectively. The mRNA levels of CB and CD were determined with real-time PCR. Results: After 12 and 24 hours, the activities of CB and CD, treated by 50mmol/L potassium iodide were decreased significanty. The mRNA levels of CB and CD of 50mmol/L potassium iodide group were decreased significanty after treatment 24 hours. Conclusion: The decreased activities of CB and CD were one of reasons of thyroid colloid retention resulted from iodine excess. |
| Keywords: | cathepsin iodine excess FRTL |
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