传统中医药在急性肺损伤中的治疗作用和机制的研究进展

急性肺损伤(acute lung injury,ALI)由于发病机制复杂,死亡率高,已成为医学领域内的一大难题.研究表明,传统中医药可以起到抗ALI作用,但其在体内的作用机制仍需进一步明确.本文对截至目前研究所得的ALI致病机制、传统中医药对ALI的治疗经验以及中西医的对比进行综述,为治疗ALI提供理论基础和临床思路,...

Research Progress on Therapeutic Effect and Mechanism of Traditional Chinese Medicine in Acute Lung Injury

OBJECTIVE To study the effect of total flavonoids from Scutellaria amoena on regulating lipid and protecting Lilver and the level of HMGCR and CYP7A1 in Hyperlipidemia Rats. METHODS Forty SPF male SD rats were divided into normal group (8 rats) and high-fat-induced group (32 rats). Rats in the normal group were fed ordinary food and others were fed high-fat food. After 8 weeks, the high-fat-induced rats were divided randomly into model group (saline), fenofibrate group (20 mg·kg-1), SAF group (100 mg·kg-1 and 20 mg·kg-1), and 1 time per day for 4 consecutive weeks. Body weights was checked every week. At the end of the experiment, the contents of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT) in serum and were measured. The histopathological changes of the liver tissues were observed by HE staining. The levels of TC, TG, HMGCR and CYP7A1 in the liver tissues were detected. RESULTS To compare with the normal group, the results showed that the model group significantly increased in body weight, and the levels of TC, TG, LDL-C, ALT and AST in serum, while HDL-C level was decreased. In liver tissue, steatosis was severe, and the levels of TC, TG and the activity of HMGCR were significantly increased, and the activity of CYP7A1 was significantly decreased (P<0.001). To compare with the model group, the results showed SAF (100 mg·kg-1, 20 mg·kg-1) groups significantly reduced the levels of TC, TG, LDL-C, AST, ALT, while significantly increased the level of LDL-C in serum (P<0.05). In liver tissue, the pathological changes were improved, and the levels of TC, TG and the activity of HMGCR were significantly reduced, while the activity of CYP7A1 was significantly increased after SAF administration. The effect was more significant in 100 mg·kg-1 group than 20 mg·kg-1. CONCLUSION The SAF can significantly reduce blood lipid level of hyperlipidemia rats, while it can improve liver function and reduce liver steatosis in hyperlipidemia rats, which suggested that it has better function of regulating lipid and protecting liver. The mechanism might be related to the down-regulation of HMGCR level to inhibit cholesterol biosynthesis and the up-regulation of CYP7A1 activity to promote cholesterol metabolism, which regulate lipid metabolism disorder.