【原创论文】前列腺组织弹性成像与间质中I、III型胶原纤维的关系

本文探讨前列腺弹性成像显示的组织硬度情况与I、III型胶原纤维的含量及二者相互走行方式的关系。对62例患者进行经直肠组织弹性成像检查（TRTE），常规12针系统穿刺活检后，对前列腺进行TRTE引导下靶向穿刺，所取组织进行天狼腥红染色，观察I、III型胶原纤维的的分布情况，并分别计算二者的容积指数（CVF）。结果得出I型胶原纤维的CVF在质硬、软组分别为：0．05±0．02和0．02±0．01；两组之间的差异显著（P=0．002）；III型胶原纤维的CVF在质硬、软组分别为：0．05±0．04与0．07±0．03；两组之间的差异不显著（P=0．13）。圆分布的统计结果表明胶原纤维在质软组和质硬组均走行紊乱，I型胶原和III型胶原之间主要呈相互交叉走行，部分呈平行走行，二者的相互走行关系在质软、质硬组之间的差异显著（P=0．03）。前列腺弹性成像显示的组织硬度主要取决于组织中I型胶原纤维的含量，另外，I型、III型胶原纤维的相互走行方式也可影响组织的弹性。

Tissue elasticity displayed by elastography and its correlation with the characteristics of collagen type I and type III in prostatic stroma

We investigated the prostate elasticity displayed by elastography and its correlation with the content and distribution of collagen type I （Col1） and type III （Col3）. A total of 62 patients underwent transrectal real-time tissue elastography （TRTE） examinations, Targeted biopsies were performed after 12-core systematic biopsy, The tissues corresponding to the elastograms were stained with picric acid-sirius red. The distribution of Col I and type Col3 was observed, and the collagen volume fraction （CVF） of these two types of collagen fibers was calculated, The CVFs of Coll in the stiff and soft groups were 0.05 ± 0.02 and 0.02 ± 0.01 {P= 0.002）, respectively. The CVFs of Col3 in the stiff and soft groups were 0.05 ± 0.04 and 0.07 ± 0,03 （P= 0,13）, respectively, The circular analysis results showed that collagen fibers were disorganized both in the soft and stiff groups, Coll and Col3 were mainly cross-linked, and some parallelization was observed in the sections. The distributions of Coll and Col3 were different between the stiff and soft groups （P = 0,03）, In conclusion, the texture of the prostate is due to the content of Coll and its relative correlation with Col3.