| B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞增殖的抑制作用 |
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| 引用本文： | 贾靖,孙立新,葛志华.B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞增殖的抑制作用[J].河北医学,2010,16(7):769-771. |
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| 作者姓名： | 贾靖 孙立新 葛志华 |
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| 作者单位： | 承德医学院附属医院,河北,承德,067000 |
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| 摘 要： | 目的：研究B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞的抑制作用。方法：制备B16F10黑素瘤细胞培养上清,作用于同基因小鼠脾淋巴细胞,通过PHA刺激和混合淋巴细胞反应,用MTT法检测淋巴细胞的增殖活性。结果：在B16F10黑素瘤细胞培养上清的作用下,PHA诱导的同基因小鼠脾淋巴细胞增殖率平均为63.00%,明显低于对照组,差异有显著性（t=16.447,p=0.000）;混合淋巴细胞反应的淋巴细胞增殖率平均为61.82%,亦明显低于对照组,差异有显著性（t=8.097,P=0.000）。结论：B16F10黑素瘤细胞培养上清对同基因小鼠脾淋巴细胞的增殖具有抑制作用。
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| 关 键 词： | 黑素瘤 淋巴细胞 增殖 PHA 混合淋巴细胞反应 |
Suppressed Proliferation of Syngenic Mouse Spleen Lymphocytes in Vitro by Supernatant from B16F10 Cell Culture |
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| JIA Jing,SUN Lixin,GE Zhihua.Suppressed Proliferation of Syngenic Mouse Spleen Lymphocytes in Vitro by Supernatant from B16F10 Cell Culture[J].Hebei Medicine,2010,16(7):769-771. |
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| Authors: | JIA Jing SUN Lixin GE Zhihua |
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| Institution: | JIA Jing,SUN Lixin,GE Zhihua(The Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China) |
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| Abstract: | Objective:To study the suppression effect on proliferation of syngenic mouse spleen lymphocytes in vitro by supernatant from B16F10 cell culture.Method: The supernatant from B16F10 cell culture was prepared and used on syngenic mouse spleen lymphocytes.The proliferations induced by PHA or MLR were determined by MTT assay.Result: Treating with supernatant from B16F10 cell culture,the proliferations of mouse spleen lymphocytes induced by PHA and MLR were 63.00% and 61.82% respectively.Both were lower than tha... |
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| Keywords: | PHA |
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